| WWP2干扰对Huh7肝癌细胞的增殖、凋亡及细胞周期的影响研究 |
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| 引用本文： | 徐胜前,秦勇,王超君,叶冠雄,吴成军,王世,潘德标,王俊.WWP2干扰对Huh7肝癌细胞的增殖、凋亡及细胞周期的影响研究[J].医学研究杂志,2017,46(11):84-87. |
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| 作者姓名： | 徐胜前 秦勇 王超君 叶冠雄 吴成军 王世 潘德标 王俊 |
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| 作者单位： | 323000 温州医科大学附属第六医院、丽水市人民医院肝胆胰外科,323000 温州医科大学附属第六医院、丽水市人民医院肝胆胰外科,323000 温州医科大学附属第六医院、丽水市人民医院肝胆胰外科,323000 温州医科大学附属第六医院、丽水市人民医院肝胆胰外科,323000 温州医科大学附属第六医院、丽水市人民医院肝胆胰外科,323000 温州医科大学附属第六医院、丽水市人民医院肝胆胰外科,323000 温州医科大学附属第六医院、丽水市人民医院肝胆胰外科,323000 温州医科大学附属第六医院、丽水市人民医院肝胆胰外科 |
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| 基金项目： | 浙江省科技厅公益应用性项目（2014C3316） |
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| 摘 要： | 目的 探讨WWP2对Huh7肝癌细胞系的增殖、凋亡及细胞周期的影响研究。方法 通过实时定量PCR （qPCR）、RNA干扰、流式细胞仪分析细胞周期及凋亡，了解WWP2在Huh7肝癌细胞周期的中作用。结果 在肝癌组织中，WWP2 mRNA水平显著性表达；敲除细胞中的WWP2会抑制细胞增殖、使细胞停滞在G1期，并诱导细胞凋亡。结论 WWP2有可能通过调控细胞凋亡而促进肝癌细胞生长。
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| 关 键 词： | WWP2干扰 Huh7肝癌细胞系 增殖 凋亡 |
| 收稿时间： | 2016/9/29 0:00:00 |
| 修稿时间： | 2017/3/14 0:00:00 |
Effects of WWP2 Interference on Proliferation, Apoptosis and Cell Cycle of Hepatocellular Carcinoma Cell Huh7 |
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| Xu Shengqian,Qin Yong,Wang Chaojun.Effects of WWP2 Interference on Proliferation, Apoptosis and Cell Cycle of Hepatocellular Carcinoma Cell Huh7[J].Journal of Medical Research,2017,46(11):84-87. |
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| Authors: | Xu Shengqian Qin Yong Wang Chaojun |
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| Institution: | Department of Hepatobiliary Surgery, People Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Zhejiang 323000, China,Department of Hepatobiliary Surgery, People Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Zhejiang 323000, China,Department of Hepatobiliary Surgery, People Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Zhejiang 323000, China,Department of Hepatobiliary Surgery, People Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Zhejiang 323000, China,Department of Hepatobiliary Surgery, People Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Zhejiang 323000, China,Department of Hepatobiliary Surgery, People Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Zhejiang 323000, China,Department of Hepatobiliary Surgery, People Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Zhejiang 323000, China and Department of Hepatobiliary Surgery, People Hospital of Lishui, The Sixth Affiliated Hospital of Wenzhou Medical University, Zhejiang 323000, China |
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| Abstract: | Objective To explore this effects of WWP2 interference on proliferation, apoptosis and cell cycle of hepatocellular carcinoma cell Huh7.Methods We used the experimental techniques of real-time quantitative PCR (qPCR), RNA interference and flow cytometry to investigate the role of WWP2 in the cell cycle of hepatocellular carcinoma (Huh7).Results WWP2 mRNA levels expressed significantly in hepatocellular carcinoma (HCC). Knock except cells of WWP2 will inhibited cell proliferation, the cell arrest in the G1 phase, and induce cell apptosis.Conclusion WWP2 may promote the growth of hepatocellular carcinoma cells through the regulation of apoptosis in vitro. |
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| Keywords: | WWP2 interference Hepatocellular carcinoma cell Huh7 Proliferation Apoptosis |
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