siRNA沉默MACC1基因表达对宫颈癌细胞增殖及侵袭的影响

目的探讨siRNA沉默结肠癌转移相关基因1(MACC1)表达对Hela宫颈癌细胞增殖及侵袭的影响。方法通过脂质体将siRNA MACC1及siRNA NC转入Hela细胞中,RT-PCR及western blot检测转染效果,MTT法检测细胞活力,流式细胞术检测细胞周期,Transwell法检测细胞侵袭能力,western blot检测细胞周期蛋白D1(cyclin D1)、cyclin E,基质金属蛋白酶2(MMP2)、MMP9蛋白表达及细胞外调节蛋白激酶(ERK)磷酸化水平。结果与siRNA NC组比较,siRNA MACC1组中MACC1蛋白及mRNA表达量降低(P0.01),细胞活力及侵袭能力下降(P0.01),细胞周期阻滞在G1期,cyclin D1、cyclin E、MMP2、MMP9及p-ERK表达量下调(P0.01)。结论siRNA MACC1能显著的抑制宫颈癌Hela细胞增殖与侵袭,可能与下调ERK磷酸化水平有关。

Effect of silencing MACC1 gene on proliferation and invasion of cervical cancer cells

Objective To explore the effect of silencing metastasis-associated in colon cancer 1 (MACC1) gene on proliferation and invasion of cervical cancer Hela cells. Methods siRNA MACC1 and siRNA NC were transfected into cervical cancer Hela cells with liposomes. The expression of MACC1 protein and mRNA was detected by western blot and RT-PCR. Cell viability and invasion ability were measured by MTT and transwell assay, respectively. The expression of cyclin D1, cyclin E matrix metalloproteinase 2 (MMP2), MMP9 and the level of extracellular regulated protein kinases (ERK) phosphorylation was detected by western blot. Results Compared with siRNA NC, the expression of MACC1 protein and mRNA was down-regulated, cell viability and invasion ability were reduced (P< 0.01), cell cycle was arrested at G1 phase (P< 0.01), the expression of cyclin D1, cyclin E, MMP2, MMP9 and p-ERK1/2 was down-regulated (P< 0.01). Conclusions siRNA MACC1 can inhibit proliferation and invasion of cervical cancer Hela cells, which migiht be related to down-regulation of p-ERK.