甲基泼尼松龙对急性期脊髓损伤bcl-2等凋亡基因的影响

【摘要】目的观察甲基泼尼松龙（methylprednisolone，MP）干预对急性期脊髓损伤凋亡相关基因bcl-2、bax、cytc和caspass-9相对表达量的影响，并探讨该药物促进脊髓功能修复的机制。方法实验动物随机分为假手术组（s组）、单纯损伤组（c组）与MP治疗组（T组）各36只，采用改良Allen’s法制作急性脊髓损伤模型，在损伤后8h、24h、3天、7天、14天和28天随机取各组6只动物处死，实时荧光定量PCR测量标本的ACt值并进行分析。结果T组bcl-2基因ACt值均小于C组，差异有统计学意义（P〈0．05）；bax基因在T组术后3天、14天和28天ACt值大于c组，差异有统计学意义（P〈0．05），T组8h、24h和7天三个时间点ACt值与C组，差异无统计学意义（P〉O．05）；T组cytc基因ACt值大于c组，差异有统计学意义（P〈0．05）；T组caspass-9基因ACt值大于c组，差异有统计学意义（P〈0．05）。结论甲强龙可以通过bcl-2、cytc及caspass-9的表达起到保护受伤节段脊髓的作用。

Effects of methylprednisolone on the neurocyte apoptotic genes such as bcl-2 following acute spinal cord injury

Objectives To observe the effects of methylprednisolone （ MP ） on the neurocyte apoptotic genes such as bcl-2, bax, cytc caspass-9 following acute spinal cord injury （ SCI ） and investigate the mechanisms of spinal cord recovery. Methods All experimental rabbits were divided randomly into 3 groups： sham group （ group S, 36 rabbits ）, control group （ group C, 36 rabbits ） and MP treatment group （ group T, 36 rabbits ）. The spinal cord injury models were made according to Allen＇s weight-drop method. 6 rabbits were selected randomly and sacrificed at 8 h, 24 h, 3 d, 7 d, 14 d and 28 d after the injury. The RTFQ- PCR test were taken according to their injured spinal sections, and the results of ACt were compared and analyzed. Results The results of ACt of gene bcl-2 in group T was lower than in group C, which is statistically significant （ P〈0.05 ）. At 3 d, 14 d and 28 d after the injury, the results of ACt of gene bax was higher in group C than in group T, which is statistically significant （ P〈0.05 ）. No significant differences were found between group C and T at 8 h, 24 h and 28 d （ P〉0.05 ）. Group T had better expressions in the ACt of gene cytc and caspass-9 than group C at all time points, which is statistically significant （ P〈0.05 ）. Conclusions MP plays a protective role in treating the acute spinal cord injury by affecting the expressions of the bcl-2, cytc and caspass-9 gene.