慢性乙型肝炎患者血清中可溶性CD40的检测及其临床意义

目的 研究慢性乙型肝炎患者血清中sCD40水平及其临床意义.方法 采用酶联免疫方法 检测176例慢性乙型肝炎患者血清中sCD40浓度,分析患者血清中sCD40浓度与临床轻重程度及肝组织不同炎症坏死分级的关系.结果 慢性乙型肝炎患者血清中sCD40浓度明显高于健康人,临床病情越重、肝组织炎症坏死越明显,sCD40浓度越高;在ALT低于80 IU/L的患者中,sCD40浓度大于80 pg/ml的患者中肝组织炎症坏死G2以上的占65.85%,明显高于sCD40浓度低于80 pg/ml的患者.结论 血清中sCD40水平与肝组织炎症坏死的程度呈正相关,可作为用于辅助判断乙肝患者病情的免疫学指标.

Level and clinical significance of soluble CD40 in patients with chronic hepatitis B

Objective To understand the level and clinical significance of soluble CD40 in patients with chronic hepatitis B. Methods Detecting the concentration of sCD40 from 176 eases with chronic hepatitis B by ELISA and analyzing its relationship with different grades of inflammation and necrosis in liver tissue. Results sCD40 from patients with chronic hepatitis B was significantly higher than those from healthy. And that the concentration of sCD40 was positive correlation with severe clinical disease and liver inflammation and necrosis. In patients whose ALT lower than 80 IU/L and sCD40 higher than 80 pg/ml, it showed that 65.85% cases have high grade of liver inflammation and necrosis, which was significantly higher than patients with sCD40 lower than 80 pg/ ml. Conclusion The concentration of sCD40 is positively related with the grade of liver inflammation and necrosis. This information could help us to evaluate the status of chronic hepatitis B as an immunological index.