亚砷酸钠对人脐静脉血管内皮细胞的毒性效应

目的探讨亚砷酸钠对体外培养的人脐静脉内皮细胞(Human umbilical vein endothelial cells,HUVECs)毒性效应的分子机制。方法通过观察细胞形态和细胞增殖实验分别观察亚砷酸钠对细胞形态及增殖能力的影响;PI染色结合流式细胞仪检测细胞周期的变化;应用实时定量RT-PCR技术检测c-Jun、c-Myc的mRNA表达水平。结果随着亚砷酸钠浓度增加,漂浮的细胞越来越多;细胞增殖抑制率越来越高;细胞周期结果显示,S期的细胞数明显减少,大多数细胞被阻滞在G0/G1期,呈典型的剂量-效应关系;亚砷酸钠下调HUVEC的c-Myc mRNAs水平。结论亚砷酸钠通过下调c-Myc基因表达,抑制细胞增殖能力,提示其在地砷病引起的血管损伤中发挥重要作用。

The toxic effects of Arsenite on the human umbilical vein endothelial cells

Objective To explore the effect of sodium arsenite on the angiogenesis to provide theoretical explanation and practical measurement for arsenism.Methods The proliferation of human umbilical vein endothelial cells(HUVECs) was used as an in vitro model to evaluate the effects of arsenic on human vascular cells.Cell proliferation was assayed by CCK-8 kit.PI staining with flow cytometry was used to detect cell cycle changes.The mRNAs were analyzed by real time quantitative RT-PCR.Results Sodium arsenite inhibited proliferation of HUVEC in a dose-dependent manner when exposed to it in levels applied.The cell number of S phase was decreased,most of the cell were blocked in G0/G1 phase.Meanwhile,the mRNAs of c-Myc were decreased after exposure to sodium arsenite.Conclusions Sodium arsenite inhibits proliferation of HUVEC through the down regulation of c-Myc,suggesting they are the major signals for arsenic to exert its suppression on the proliferation of HUV-EC.