萝卜硫素的体外抗氧化和抑菌活性

目的观察萝卜硫素(SFN)体外抗氧化和抑菌活性。方法试剂盒检测SFN总抗氧化能力(TAC)、抑制超氧阴离子(O2-)活力;水杨酸比色法和邻苯三酚自氧化法检测羟自由基(OH-)与超氧阴离子(O2-)清除能力;滤纸片法检测SFN的抑菌作用,连续二倍稀释法测定其最低抑菌浓度(MIC)、最低杀菌浓度(MBC)和对大肠杆菌和金色葡球菌生长的抑制。结果 SFN在体外模拟系统中总抗氧化活性和抑制抑制O2-生成活力较高,但对OH-和O2-的直接清除率较低,低于30%。抑菌结果表明SFN对大肠杆菌、金色葡萄球菌、绿脓杆菌、枯草杆菌、表皮葡萄球菌5种受试菌均有较强的抑制作用,MIC为0.78～6.25μg/ml之间。结论 SFN具有较好的抗氧化活性和较强的抑菌效果。

In vitro studies on antioxidant and antimicrobial activities of sulforaphane

Objective To investigate the antioxidation and antimicrobial activity of sulforaphane.Methods Antimicrobial diameters to bacteria and molds were assayed with filter paper;the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) values of sulforaphane were detected by serial two-fold dilution method;and the antioxidant effect of sulforaphane was studied according to total antioxidant capacity(TAC),the ability to scavenge superoxide anion radicals(O-2) and hydroxyl radicals(OH-) were observed by salicylic acid colorimetry and pyrogallol autoxidation method.Results The different concentration of sulforaphane scavenge O-2 and OH-under 30%,with the TAC and the resisting ability exceed to 160 U/ml.MIC of sulforaphane against the E.coli,S.aur,P.aer,B.sub,S.epi was 0.78～6.25 μg /ml.Conclusions Sulforaphane seems has good prospect of exploitation and utilization.