ROSCO药片扩散法对白色念珠菌体外抑菌研究

目的了解抗真菌药物的体外活性现状,为选择药敏试验方法和合理使用抗真菌药物提供依据。方法临床送检标本用TTC-沙保罗琼脂平板分离培养,常规法或API20CAUX酵母样真菌鉴定试条进行鉴定；ROSCO酵母样真菌药敏药片扩散法进行药敏试验。结果196株白色念珠菌对特比奈酚、伊曲霉素、氟胞嘧啶、益康唑、咪康唑、克霉唑、酮康唑、氟康唑、两性霉素B、游霉素的耐药率分别为71．4％、47．9％、34．6％、38．7％、39．7％、27．5％、29．5％、29．5％、10．2％、2．0％。结论白色念珠菌对游霉素和两性霉素B敏感性高,对特比奈酚和伊曲霉素耐药性高,咪唑组和三唑组吡咯类近30％～40％耐药程度；提示临床不能再靠经验用药,要参考药敏试验结果合理用药。

Study on In-vitro Activity of 10 Anti-Fungal Drugs against Candida Albicans by Using ROSCO K-B Method

Objective To study the clinical practice of the drug sensitivity test of candida albicans and the current situation of in-vitro activity of 10 anti-fungal drugs by using ROSCO K-B method,and provide scien- tific basis for rational application of methodology of drug sensitivity tests and application of anti-fungal drugs.Me- thods Clinical specimen were isolated with TTC-Sabouroud agar plate.Identification of bacteria was performed by using AP120 CAUX yeast-lide colony's identification and diagnostic discs.The drug sensitivity tests of fungals were conducted by applying ROSCO K-B method.Results Among 196 candida albicans strains,the drug resis- tance to terbinafine,itraconazole,flucytosin,econazole,miconazole,clotrimazole,ketoconazole,fluconazole,ampho- tericin B and natamycin was 71.4%,47.9%,34.6%,38.7%,39.7%,27.5%,29.5%,29.5%,10.2% and 2.0%,respectiv- ely.Conclusion Candida albicans showed a high sensitivity to natamycin and amphotericin B,whereas it showed a high resistance to terbinafine and itraconazole.The resistance reached approximately 30%-40% to group imidaz- ole and group triazole.The above results suggested that clinical application ofanti-fungal drugs should refer to the drug sensitivity tests and empirical remedy should be avoided.