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| Neuritin在毕赤酵母中的分泌表达与鉴定 | |
| 引用本文： | 张树军,仙玲玲,于娜,巴鹏飞,徐芬,杨磊,黄瑾. Neuritin在毕赤酵母中的分泌表达与鉴定[J]. 农垦医学, 2006, 28(5): 321-324 |
| 作者姓名： | 张树军 仙玲玲 于娜 巴鹏飞 徐芬 杨磊 黄瑾 |
| 作者单位： | 石河子大学医学院新疆地方与民族高发病重点实验室,新疆,石河子,832002;石河子大学医学院新疆地方与民族高发病重点实验室,新疆,石河子,832002;石河子大学医学院新疆地方与民族高发病重点实验室,新疆,石河子,832002;石河子大学医学院新疆地方与民族高发病重点实验室,新疆,石河子,832002;石河子大学医学院新疆地方与民族高发病重点实验室,新疆,石河子,832002;石河子大学医学院新疆地方与民族高发病重点实验室,新疆,石河子,832002;石河子大学医学院新疆地方与民族高发病重点实验室,新疆,石河子,832002 |
| 摘 要： | 目的：构建Neuritin的毕赤酵母分泌表达系统，为进一步探讨Neuritin的生物学功能及作用机制奠定基础。方法和结果：在巳克隆Neuritin cDNA的基础上，PCR扩增出Neuritin ORF与载体pPIC9k重组，然后利用电击转化将重组质粒pPIC9k—Neuritin导入GSll5酵母菌株。筛选出阳性克隆经PCR及测序正确后，用甲醇诱导Neuritin分泌表达；表达产物经SDS—PAGE及Western—blot鉴定。结论：成功构建Neuritin的毕赤酵母分泌表达系统。 |
| 关 键 词： | Neuritin pPIC9k 巴斯德毕赤酵母 基因表达 |
Secretary Expression and Identification of Neuritin in Pichia Pastoris |
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| Zhang Shujun,Xian Lingling,Yu Na,Ba Pengfei,Xu Fen,Yang Lei,Huang Jin. Secretary Expression and Identification of Neuritin in Pichia Pastoris[J]. Agricultural Reclamation Medicine, 2006, 28(5): 321-324 | |
| Authors: | Zhang Shujun Xian Lingling Yu Na Ba Pengfei Xu Fen Yang Lei Huang Jin |
| Affiliation: | Cooperation Lab of Province Ministry of Education Key Lahoratory of Xinjiang Endemic and Ethnic Disease, Xinjiang Shihezi 832002,China |
| Abstract: | Objective: Construct Pichia pastoris secretory system of Neuritin,set a base for making a further research on function and mechanism of Neuritin.Methods and results:We amplified Neuritin open reading frame from cloned Neuritin cDNA by PCR and inserted it into pPIC9k vector,then transform pPIC9k-Neuritin recombinant into Pichia pastoris GS115.After identifying positive clone by PCR analysis and sequencing,the recombinant was induced by methanol to express Neuritin.The expressed products were analysised by SDS-PAGE and Western-blot.Conclusion:We successfully constructed Pichia pastoris secretory system of Neuritin. |
| Keywords: | Neuritin pPIC9k |
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