| 大鼠耳蜗血管纹内皮细胞的原代培养 |
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| 引用本文： | 韦嘉章,苏纪平,冯爽. 大鼠耳蜗血管纹内皮细胞的原代培养[J]. 广西医科大学学报, 2009, 26(1): 7-9 |
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| 作者姓名： | 韦嘉章 苏纪平 冯爽 |
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| 作者单位： | 广西医科大学第一附属医院耳鼻咽喉头颈外科,南宁,530021;广西医科大学第一附属医院耳鼻咽喉头颈外科,南宁,530021;广西医科大学第一附属医院耳鼻咽喉头颈外科,南宁,530021 |
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| 基金项目： | 广西自治区科学基金,教育部留学回国人员科研启动基金,广西研究生教育创新计划 |
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| 摘 要： | 目的：原代培养大鼠耳蜗血管纹血管内皮细胞,为血迷路屏障研究提供体外细胞模型。方法：显微解剖分离新生Wistar大鼠耳蜗血管纹组织,运用胰蛋白酶消化与机械分离组织块相结合的方法培养细胞,免疫组织化学染色鉴别细胞来源。结果：部分血管纹组织块培养48h后,自边缘生长出零散的细胞,逐渐增殖并形成致密融合的单层多边形细胞的细胞集落,经传代纯化的细胞具有内皮细胞培养典型的＂铺路石样＂外观,免疫组织化学检测内皮细胞标志性抗原Ⅷ因子,细胞胞浆中呈棕黄色阳性反应。结论：本实验培养出大鼠耳蜗血管纹血管内皮细胞,为血迷路屏障体外研究提供了细胞模型。
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| 关 键 词： | 血迷路屏障 细胞模型 原代培养 大鼠 |
PRIMARY CULTURE OF COCHLEAR ENDOTHELIAL CELLS FROM STRIA VASCULARIS OF RATS |
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| Wei Jiazhang,Su Jiping,Feng Shuang. PRIMARY CULTURE OF COCHLEAR ENDOTHELIAL CELLS FROM STRIA VASCULARIS OF RATS[J]. Journal of Guangxi Medical University, 2009, 26(1): 7-9 |
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| Authors: | Wei Jiazhang Su Jiping Feng Shuang |
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| Affiliation: | Wei Jiazhang,Su Jiping,Feng Shuang.(Department of Otolaryngology-Head and Neck Surgery,First Affiliated Hospital,Guangxi Medical University,Nanning 530021 China) |
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| Abstract: | Objective:To provide an invitro cell model for blood-labyrinth barrier by primary culturing cochlear capillary endothelial cells from stria vascularis of rats. Methods:Cochlear stria vascularis were acquired from neonatal Wistar rats with micrergy,the cells were cultured by trypsin digestion with tissue nubbles cultivation,then the immunocytochemistry was employed to classify cell types.Result:48 hours after tissue nubbles cultivation of stria vascularis,a few cells were seen disseminately around partial ti... |
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| Keywords: | blood-labyrinth barrier cell model primary culture rats |
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