缬草挥发油与水提物镇静催眠作用比较研究

目的：了解缬草镇静催眠的主要药效物质，为深入研究其有效成分提供物质基础。方法：通过缬草挥发油急性毒性实验，了解挥发油毒性的大小，确定药效实验剂量。采用镇静催眠的药效学实验，比较缬草挥发油与水提物协同戊巴比妥钠对小鼠的催眠作用。结果：小鼠灌胃缬草挥发油的LD50为278．99g·kg^-1（以生药计），从而确定药效试验的给药剂量为高剂量组（85．71g·kg^-1）、中剂量组（57．14g·kg^-1）和低剂量组（28．57g·kg^-1）；缬草镇静催眠药效学实验表明，缬草挥发油可促进小鼠入睡效率，延长小鼠睡眠时间；水提物仅可延长小鼠的睡眠时间，与水提物相比，缬草挥发油能有效延长小鼠的睡眠时间。结论：缬草挥发油的镇静催眠效果明显优于其水溶性物质。

Comparative Study on Sedative and Hypnotic Effects of Volatile Oil and Water Extract from Valerian

Objective:To understand the primary materials from valerian with sedative and hypnotic effects to provide basis for further research on the effective component.Method:Through the acute toxicity test,the toxicity of valerian volatile oil could be detected to decide the dosage in the efficacy trials.Through the sedative and hypnotic experiment,the cooperative effects of valerian volatile oil and water extract with pentobarbital sodium in mice were studied and compared.Result:The LD_(50) of valerian volatile o...