mfn2基因在猫爪草皂苷、红三叶异黄酮治疗乳腺癌中的作用机制研究

目的：研究mfn2基因在猫爪草皂苷、红三叶异黄酮抑制人乳腺癌MCF-7细胞增殖及诱导其凋亡中的作用。方法：采用不同浓度的猫爪草皂苷和红三叶异黄酮分别处理MCF-7细胞24，48及72h，进行体外细胞培养，用实时荧光定量PCR法检测mfn2基因的相对表达情况。结果：高浓度的猫爪草皂苷（100mg·L^-1）作用MCF-7细胞48h后，明显促进mfn2基因的表达，并且随时间延长更加明显；红三叶异黄酮在作用MCF-7细胞48，72h后，显著抑制mfn2基因的表达。结论：高浓度的猫爪草皂苷可能是通过促进mfn2基因的表达，来抑制MCF-7细胞增殖及诱导其凋亡，红三叶异黄酮的作用机制有待于进一步研究。

Mechanism Study of mfn2 Gene in Radix Ranuncoli Ternati Saponins and Red Clover Isoflavone treated Breast Cancer

Objective： To study the mechanism of infn2 gene in radix ranuncoli ternati （RRTS） and red clover isoflavone-treated breast cancer. Method： MCF-7 cells were cuhrued in vitro, then separately treated by different concentration of RRTS （25, 50 and 100 mg· L^- 1 ） and red clover isoflavone （ 50,100 and 200 μg· ml^- 1 ） for 24, 48 and 72 h. The total RNA of each sample was extracted by Tfizol method. The relative expression of mfn2 gene was measured by real-time PCR. Result： There was no significant difference in the expression of mfn2 gene between the control group and low concentration group （25 and 50 mg· L^- 1 ） of RRTS, however the high concentration （100 mg· L^- 1） of RRTS could obviously promote the expression of mfn2 gene in MCF-7 ceils treated for 48 h, and the expression was increased with the treating time. On the contrary, red clover isoflavone inhibited the expression after treating MCF-7 cells for 48 h and 72 h （P 〈0. 01 ）. Conclusion： High concentration of RRTS （ 100mg· L^- 1 ） can inhibit the proliferation and promote the apoptosis of MCF-7 cells through affecting mfn2 gene while the mechanism of red clover isoflavone needs further study.