|
|||||
|
|
Cross-reactivity between solid-phase immunoassay plates and intermediate filaments demonstrated by human monoclonal antibodies |
|
| Authors: | D O Haskard V Gul J R Archer |
| Affiliation: | The ARC Bone and Joint Research Unit, The London Hospital Medical College, 25 - 29 Ashfield Street, London E1 2AD, U.K. |
| Abstract: | While screening supernatants of human-human hybridomas for rheumatoid factor and anti-cellular activity we found that a significant number of supernatants which react with the Falcon-polyvinyl chloride immunoassay plate used in an enzyme-linked immunosorbent rheumatoid factor assay also react with intracellular intermediate filaments. |
| Keywords: | monoclonal antibody cross-reactions intermediate filaments solid-phase immunoassay ELISA enzyme-linked immunosorbent assay BSA bovine serum albumin HEp 2 laryngeal carcinoma cells PBS phosphate-buffere saline PBS-Tween phosphate-buffered saline containing 0.05% Tween 20 |
| 本文献已被 ScienceDirect 等数据库收录！ | |