ROLE OF INTERMEDIARY BIOMARKERS IN DETERMINING THE ANTICANCER EFFICACY OF MARINE COMPOUNDS |
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| Authors: | Jamal M. Arif Mohammed Kunhi Yunus M. Siddiqui Khalid A. El Sayed Khaled Y. Orabi Amal Al-Hazzani Mohammed N. Al-Ahdal Fahad M. Al-Khodairy |
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| Affiliation: | (1) Biological and Medical Research, King Faisal Specialist Hospital and Research Institute, P.O. Box 3354, Riyadh, Saudi Arabia, 11211;(2) Department of Basic Pharmaceutical Sciences, School of Pharmacy, University of Louisiana at Monroe, 700 University Avenue, Monroe, Louisiana 71209, USA;(3) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kuwait University, Safat, Kuwait, 13110 |
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| Abstract: | In the present study, two of the probable an umor marine compounds, manzamine A and sarcophine, were screened using benzo[a]pyrene (BP)-derived DNA adduct formation in MCF-7 cells as intermediary biomarker. Briefly, MCF-7 cells were treated with the compounds for 24 h followed by treatment with BP (0.5 μM). After 24h incubation, cellular DNA was isolated and analyzed for BP-derived DNA adducts by 32P-postlabeling technique. Manzamine A and sarcophine increased the BP-DNA adducts by 2 to 4-folds. Further, manzamine A (50 μM) substantially down regulated the expression of p53 while sarcophine (50 μM) slightly induced the level of p21. The residual DNA repair ability was almost completely abolished by manzamine A while sarcophine was ineffective. Based on our preliminary results, these compounds may be classified as potential genotoxic. |
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