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| Notch信号分子在ICA诱导小鼠胚胎干细胞向心肌细胞分化的作用 | |
| 引用本文： | 马微,;刘光耀,;杨国宏,;刘春玲,;闫磊,;黄永纯,;孙晓冬. Notch信号分子在ICA诱导小鼠胚胎干细胞向心肌细胞分化的作用[J]. 解剖科学进展, 2014, 0(5): 443-445 |
| 作者姓名： | 马微, 刘光耀, 杨国宏, 刘春玲, 闫磊, 黄永纯, 孙晓冬 |
| 作者单位： | [1]牡丹江医学院组织学与胚胎学教研室,黑龙江牡丹江157011; [2]牡丹江医学院,研究生处,黑龙江牡丹江157011; [3]牡丹江医学院附属红旗医院,黑龙江牡丹江157011 |
| 基金项目： | 黑龙江省教育厅研究生创新科研项目（No.YJSCX2012-381HLJ）;牡丹江医学院科学技术研究项目（No.2011-03） |
| 摘 要： | 目的观察Notch信号通路在淫羊藿苷(ICA)诱导胚胎干细胞(ESC)向心肌细胞分化过程中的作用。方法实验分为自然分化组、ICA诱导分化组及ICA+MW167(Notch信号抑制剂)组。相差显微镜下观察各组自发性节律收缩细胞出现情况的差别,免疫荧光染色观察各组细胞心肌特异性蛋白ANP的表达,RT-PCR检测各组细胞ANP mRNA的表达。结果 ICA+MW167组自发性节律收缩细胞数明显多于自然分化组与ICA诱导分化组,ICA+MW167组心肌细胞特异性蛋白ANPmRNA和蛋白的表达水平随培养时间延长逐渐增加,明显高于自然分化组与ICA诱导分化组。结论 Notch信号通路参与了ICA诱导ESC分化为心肌细胞的过程,抑制该信号通路可以明显提高心肌细胞特异性ANP基因及蛋白的表达,上调分化的心肌细胞比率。 |
| 关 键 词： | 胚胎干细胞 心肌细胞 淫羊藿苷 Notch信号分子 小鼠 |
The action of Notch signaling molecule in differentiation of murine embryonic stem cells into cardiomyocyte induced by icarrin |
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| Affiliation: | MA Wei, LIU Guang-yao, YANG Guo-hong, LIU Chun-ling, YAN Lei, HUANG Yong-chun, SUN Xiao-dong ( 1. Department of Histology and Embryolog3, and 2. Department of Graduate Students, and 3.Hongqi Hospital, Mudanjiang Medical College, Heilongjiang Mudanjiang 157011, China ) |
| Abstract: | Objective To study the action of Notch signaling molecule in differentiation of murine embryonic stem cells（ESCs） into cardiomyocyte induced 3y icarrin. Methods The murine ESCs（ES-EI4） were divided into three groups： control, ICA group and ICA＋MW167（Notch inhibition）. The expression of ANP protein and mRNA in ESCs was determined by immunofluorescenee staining and RT-PCR respectively. Results The number of the beating ESCs was significantly increased in ICA＋MW167 group than in ICA group and in control group. The expression levels of ANP protein and mRNA in ESCs were higher in ICA＋MW167 group than in ICA group and in control group. Conclusion MW167 can promote the differentiation of embryonic stem ceils to cardiomyoeytes. |
| Keywords: | embryonic stem cell cardiomyocyte icariin Notch molecule murine |
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