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| He-Ne激光照射对脑梗死患者淋巴细胞生物学的影响 | |
| 引用本文： | 朱键,梁敏仪,刘承宜.He-Ne激光照射对脑梗死患者淋巴细胞生物学的影响[J].中国激光医学杂志,2008,17(4). |
| 作者姓名： | 朱键 梁敏仪 刘承宜 |
| 作者单位： | 1. 广州医学院附属第一医院激光室,广州市,510120 2. 广州医学院医学遗传学与细胞生物学教研室 3. 华南师范大学激光运动医学实验室 |
| 基金项目： | 广东省卫生厅科研项目，广州医学院附属第一医院基金 |
| 摘 要： | 目的 探讨鼻腔内低强度激光照射治疗(ILILT)对脑梗死患者淋巴细胞增殖、畸变、凋亡和细胞周期动力学指数的影响,以及致突变性.方法 对ILILT前、后的35例脑梗死患者和35例健康成年人外周血进行淋巴细胞培养,并进行细胞增殖指数(MI)、细胞周期动力学指数(CKI)、姐妹染色单体交换(SCE)频率和细胞凋亡的分析,观察ILILT对淋巴细胞的效应.结果 ILILT后,脑梗死患者的淋巴细胞增殖活性比对照组和照射前有所提高,并且随着照射剂量的增加其增殖活性也增高(P＜0.05,P＜0.001),但对淋巴细胞周期的进程影响不大.各组间的SCE频率差异不显著(P＞0.05).ILILT前、后的CKI与对照组相比,差异无显著意义(P＞0.05).ILILT后脑梗死患者的细胞凋亡率比照射前和对照组比较均有极显著增加(P＜0.001).结论 ILILT能提高脑梗死患者的淋巴细胞增殖活性,虽可诱发较高的淋巴细胞凋亡率,但对细胞没有致突变性. |
| 关 键 词： | 激光 脑梗死 淋巴细胞 |
Effects of He-Ne Laser Irradiation on Lymphocytes Proliferation,Aberration,Apoptosis and Cell Cycle Kinetic Index in Patients with Cerebral Infarction |
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| ZHU Jian,LIANG Min-yi,LIU Cheng-yi.Effects of He-Ne Laser Irradiation on Lymphocytes Proliferation,Aberration,Apoptosis and Cell Cycle Kinetic Index in Patients with Cerebral Infarction[J].Chinese Journal of Laser Medicine & Surgery,2008,17(4). | |
| Authors: | ZHU Jian LIANG Min-yi LIU Cheng-yi |
| Institution: | ZHU Jian1,LIANG Min-yi2,LIU Cheng-yi3 Department of Laser Medicine,the First Hospital,Guangzhou Medical College,Guangzhou,510120 China 2.Department of Medical Genetics , Biology,3 Laboratory of Laser Sports Medicine,South China Normal University |
| Abstract: | Objective To explore the effects of intranasal low intensity laser therapy(ILILT) on cell proliferation,aberration, apoptosis and genotoxicity of lymphocytes in patients with cerebral infarction(CI). Methods The lymphocytes in peripheral blood in patients with CI were sampled before and after treatment and cultured, and then some parameters such as the mitosis index(MI), cell cycle kinetic index(CKI), frequency of sister chromatid exchanges (SCE) and apoptosis were analyzed in order to explore the effects o... |
| Keywords: | Laser Cerebral infarction Lymphocyte |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |