黄芪提取物对体外高糖培养牛视网膜毛细血管周细胞的影响

目的:观察中药黄芪提取物对体外高糖培养牛视网膜毛细血管周细胞(pericyte,PC)的影响.方法:在体外高糖(20mmol/L)培养2d的第3代周细胞中加入不同浓度的黄芪提取物,继续培养2d,用MTT比色法,测定每组的吸光度值(A值),根据A值计算抑制率.另在周细胞中加入0.4g/L黄芪提取物,2d后收集细胞,用流式细胞仪测定凋亡细胞百分率.结果:黄芪提取物0.0256～10 000 mg/L浓度组对高糖培养周细胞的增殖有促进作用,其A值与空白对照组相比有显著性差异(P＜0.05).在周细胞以20mmol/L葡萄糖培养48h后,加入400mg/L黄芪提取物组的细胞凋亡百分率低于空白对照组和模型组(12.2%±1.5% vs 7.30%±2.0%,18.6%±2.1%P＜0.05).结论:黄芪能抑制周细胞凋亡,提高体外高糖培养视网膜毛细血管周细胞的存活力.

Effect of Milkvetch to bovine retinal capillary pericytes cultured with high glucose in vitro

AIM:To observe the effect of Traditional Chinese Medicine extractive of Milkvetch Root to bovine retinal capillary pericytes(PC) .METHODS:Different concentration extractives of Milkvetch Root were put in the pericytes of third generation which cultured with high glucose 2 days in vitro,and then the OD of living cells was measured by MTT for another 24 hours.According to the OD,the ratio of suppressing cells could be calculated.Extractives of Milkvetch Root(0.4g/L) were put in the pericytes of third generation which cultured with high glucose 2 days in vitro.After cultured with high glucose(20mmol/L) in vitro 2 days,PC cells were collected and measured the percent of apoptosis cells in PC by Flow Cytometry.RESULTS:The groups of 1-10 concentration could promote pericytes proliferation,and the OD was different with the control group(P <0.05) .After cultured with 20mmol/L glucose for 2 days,the apoptosis percent of PC in 0.4g/L Chinese medicine was lower than control or model groups(P<0.01) .CONCLUSION:Milkvetch Root can suppress the apoptosis of PC,promote the survival ability of PC cultured in vitro,and consequently it can protect PC.