RP-HPLC法同时测定细辛中甲基丁香酚、黄樟醚和细辛脂素的含量

目的:建立同时测定细辛中甲基丁香酚、黄樟醚、细辛脂素含量的HPLC方法。方法:采用Agilent Eclipse XDB-C18(4.6 mm×150 mm,5"m)色谱柱,流动相为甲醇-水,梯度洗脱,流速1.0 mL·min-1,检测波长285 nm,柱温35℃。结果:甲基丁香酚、黄樟醚和细辛脂素的进样量分别在0.10～2.07μg、0.11～2.20μg和0.054～1.07μg内线性关系良好(r=0.999 9)。平均回收率(n=6)分别为98.4%,97.7%,98.6%;RSD分别为0.78%,0.41%,1.5%。结论:该方法简便、可行,重现性好,为细辛的质量控制提供参考。

RP-HPLC simultaneous determination of methyleugenol,safrole andasarinin in Asari Radix et Rhizoma

Objective:To develop an HPLC method for determination of methyleugenol,safrole and asarinin in Asari Radix et Rhizoma.Methods: Agilent Eclipse XDB-C18(4.6 mm×150 mm,5 μm) column was adopted,the mobile phase consisted of methanol-water at the flow rate of 1.0 mL·min-1,the detective wavelength was 285 nm,and the column temperature was 35 ℃.Results: Methyleugenol,safrole and asarinin showed good linearity(r=0.9999) in the ranges of 0.10-2.07 μg,0.11-2.20 μg and 0.054-1.09 μg.The recovery rates(n=6) of methyleugenol,safrole and asarinin were 98.4%,97.7% and 98.6%,and RSD were 0.78%,0.41%,1.5%,respectively.Conclusion: This method is simple and can be repeated easily,and can be used for the quality control of Asari Radix et Rhizoma.