脐血DC-CIK共培养体外抗肿瘤效应及趋化性的实验研究

目的探讨细胞因子诱导的杀伤细胞（CIK）与同源树突状细胞（DC）共培养后对人白血病K562细胞、人淋巴瘤raji细胞、人乳腺癌MCF-7细胞的杀伤作用及CIK细胞的趋化性。方法采集健康产妇分娩的正常足月胎儿脐血,分离单个核细胞,诱导培养CIK、DC细胞。将成熟DC和CIK混合培养3d,用MTT法检测CIK、DC-CIK对K562、raji、MCF-7细胞的杀伤活性;趋化试验检测CIK细胞的趋化性。结果 CIK、DC-CIK细胞对K562、raji、MCF-7细胞均具有较强的杀伤作用,DC-CIK杀伤活性明显高于CIK。趋化试验显示,IL-8、MCP-1作用后穿过微孔滤膜的细胞数明显高于阴性对照。结论 DC-CIK共培养可明显提高CIK对K562、raji、MCF-7细胞的杀伤作用,IL-8、MCP-1对CIK细胞存在趋化性。

Experimental study for antitumor effective and chemotaxis of co-cultured DC and CIK from cord blood in vitro

Objective To observe the cytotoxicity on the K562,raji,MCF-7 cells and chemotaxis of cytokine-induced killer cells （ CIK） which co-cultured with homologous dendritic cells （ DC） . Methods Cord blood from term fetus delivered by healthy puerpera was collected,the mononuclear cells were isolated and CIKcells,DCcells were induced. The matured CIK and DC cells were mixed for 3d,the killing activity of CIK cells and DC-CIK cells on K562,raji,MCF-7 cell were detectd by using MTT assay ; the chemotaxis of CIK was detectd by using the chemotactic experiment. Results CIK, DC-CIK cells showed strong killing effect sto K562,raji,MCF-7 cells,and the killing activity of DC-CIK was significantly higher than that of CIK. Chemotaxis experiment showed that the number of the cells which passed through the microporous treated after IL-8,MCP-1was significantly higher than that in the negative control. Conclusion Co-culture of CIK with DC can improve the cytotoxicity to the K562,raji,MCF-7 cells; IL-8,MCP-1 have chemotaxis to CIK cells.