免疫透射比浊法测定肿瘤特异性生长因子方法学评价

对肿瘤特异性生长因子（TSGF）方法学和临床应用进行评价，采用免疫透射比浊法与微粒子酶免疫法（MEIA）进行比较，对其重复性、线性等进行分析。用本法与MEIA法检测120份血清标本TSGF浓度，结果经χ^2检验，无显著性差异（P〉0．05），与MEIA法比较阳性符合率为95．7％，阴性符合率为96％，总符合率为95．8％；低、高浓度的批内CV为3．8％和4．2％，批间CV为4．0％和4．9％；检测浓度小于64U／mL时，线性良好，Y=0．899X＋1．876，r=0．9882；RF、SLE和炎症患者血清和重度溶血、脂血、黄疸血清等对检测均有～定的影响。该TSGF方法符合临床常规实验室检测的要求。

Evaluation of Detecting TSGF Levels by Immunoturbidimetric Method

To evaluate the tumor specific growth factor （TSGF） levels detection method and its clinical application. The immunoturbidimetric method was compared with the technique of MEIA, and the performance characteristics such as reproducibility, linearity, influential factors of TSGF were analyzed. The TSGF levels in 120 serum samples were detected by both methods. The results showed that there was no significant difference between two methods by chi-square（χ^2） test （P 〉 0.05）. The positive accordant ratio was 95.7%; the negative one was 96.0% ; and the total accordant ratio was 95.8% The inter and intra CVs of the serum TSGF levels with low and high value were 3.8% , 4.2% and 4.0% , 4.9% ,respectively. The linearity was good when detection level was less than 64U/mL （ Y = 0.899X ＋ 1. 8759, r = 0. 9882） ; The serum of positive RF, SLE and high level of hemoglobin, bilirubin and lipemia had influence on the detection of TSGF levels. The detection of TSGF by immunoturbidimetric method may still fulfill the requirements of clinical laboratory.