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小桐子抑菌成分粗提工艺及其活性研究
引用本文:伍保龙,易弋,夏杰,廖春文,田玉红. 小桐子抑菌成分粗提工艺及其活性研究[J]. 时珍国医国药, 2012, 23(8): 1907-1909
作者姓名:伍保龙  易弋  夏杰  廖春文  田玉红
作者单位:1. 广西工学院,广西柳州,545006
2. 深圳市东域生物科技有限公司,广东深圳,518000
基金项目:广西壮族自治区自然科学基金青年基金(No.0991010)
摘    要:目的 探索小桐子综合开发利用的价值.方法 在不同条件下对小桐子树叶中的抑菌成分进行粗提,以好食脉孢霉和粗糙脉孢霉为对象,检验提取物的最低抑菌浓度(MIC).结果 最佳提取工艺为:纯水作为提取液,料液比为1:15,30℃恒温搅拌提取10 h,此时提取物对供试菌的最低抑菌浓度为1.0g/L,粗提液经醇沉后,最低抑菌浓度下降为0.5 g/L.结论 小桐子提取物有较强的抑菌活性,在植物源抑菌剂的开发方面具有良好的应用前景.

关 键 词:小桐子  抑菌  粗糙脉胞霉  好食脉胞霉

Crude Extract of Antibacterial Components from Jatropha eurcas and its Antibacterial Activity
WU Bao-long , YI Yi , XIA Jie , LIAO Chun-wen , TIAN Yu-hong. Crude Extract of Antibacterial Components from Jatropha eurcas and its Antibacterial Activity[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(8): 1907-1909
Authors:WU Bao-long    YI Yi    XIA Jie    LIAO Chun-wen    TIAN Yu-hong
Affiliation:1 (1.Guangxi University of Technology,Liuzhou 545006,China;2.Shenzhen Dongyu Biotechnological Co.,Ltd.Shenzhen,Guangdong 518000,China)
Abstract:Objective To explore the application value of Jatropha eurcas.Methods The crude antibacterial components of the leaves of Jatropha eurcas was extracted under different conditions,then the MICE of the extracts against Neurospora sitophila and Neurospora crassa was investigated.Results The optimized extraction conditions were:water was used as extraction solvent,the rate of material to solvent was 1:15,temperature for extraction was 30℃ for 10 h.Under these conditions,the MIC of extracts against N.sitophila and N.crassa were both 1.0 g/L.When the extracts was processed by ethanol precipitation,the MIC was 0.5 g/L.Conclusion The crude extraction of the leaves of Jatropha eurcas shows a strong antibacterial activity,and has a good prospect to produce bacteriostat.
Keywords:Jatropha eurcas  Antibacterial  Neurospora sitophila  Neurospora crassa
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