| 慢性再生障碍性贫血患者中医证型与外周血microRNA、淋巴细胞亚群及细胞因子的关系研究 |
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| 引用本文： | 李峻,陈劼,孙雪梅,朱学军,姜鹏君,陈健一,倪海雯,张文曦,夏雯.慢性再生障碍性贫血患者中医证型与外周血microRNA、淋巴细胞亚群及细胞因子的关系研究[J].上海中医药大学学报,2017,31(5):16-21. |
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| 作者姓名： | 李峻 陈劼 孙雪梅 朱学军 姜鹏君 陈健一 倪海雯 张文曦 夏雯 |
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| 作者单位： | 南京中医药大学附属医院江苏省中医院血液科江苏南京210029,南京中医药大学附属医院江苏省中医院病理科江苏南京210029,南京中医药大学附属医院江苏省中医院血液科江苏南京210029,南京中医药大学附属医院江苏省中医院血液科江苏南京210029,南京中医药大学附属医院江苏省中医院血液科江苏南京210029,南京中医药大学附属医院江苏省中医院血液科江苏南京210029,南京中医药大学附属医院江苏省中医院血液科江苏南京210029,南京中医药大学附属医院江苏省中医院血液科江苏南京210029,南京中医药大学附属医院江苏省中医院血液科江苏南京210029 |
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| 基金项目： | 江苏省自然科学基金资助项目(BK20131034)；江苏省政府留学奖学金资助项目苏教外(2015)44号；江苏省卫生国际(地区)交流支撑计划青年医师自选项目苏卫国合(2016)155号 |
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| 摘 要： | 目的:分析慢性再生障碍性贫血(CAA)患者外周血microRNA、淋巴细胞亚群、细胞因子水平与不同肾虚证型的关系。方法:纳入60例CAA患者,辨证分为肾阴虚组30例、肾阳虚组14例、肾阴阳两虚组16例,另选择20例健康志愿者作为正常对照组。采用实时荧光定量PCR方法检测受试者外周血单个核细胞miRNA-155-5p、miRNA-1260b表达情况,采用流式细胞术检测外周血淋巴细胞亚群包括总T淋巴细胞(T)、辅助T淋巴细胞(Th)、细胞毒T淋巴细胞(Tc)、B淋巴细胞(B)、自然杀伤细胞(NK)],采用酶联免疫吸附法检测血浆干扰素-γ(IFN-γ)、白介素-12(IL-12)p70、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)水平。结果:与正常对照组比较,肾阴虚组、肾阴阳两虚组、肾阳虚组患者的miRNA-155-5p、miRNA-1260b表达及Tc比例、IFN-γ、IL-12 p70、TNF-α水平均明显升高(P0.01,P0.05),NK细胞比例、Th比例、Th/Tc比值及TGF-β1水平均明显降低(P0.05,P0.01)。与肾阳虚组比较,肾阴虚组、肾阴阳两虚组患者的miRNA-155-5p、miRNA-1260b表达及Tc比例、IFN-γ、IL-12 p70、TNF-α水平均明显升高(P0.01,P0.05),Th比例、Th/Tc比值及TGF-β1水平均明显降低(P0.05,P0.01);肾阴虚组患者的Th比例低于肾阴阳两虚组(P0.05),血浆IFN-γ、IL-12 p70、TNF-α水平高于肾阴阳两虚组(P0.05)。结论:肾阴虚型、肾阴阳两虚型较肾阳虚型患者存在更严重的细胞免疫功能亢进及miRNA-155-5p、miRNA-1260b表达失调,而肾阴虚型较肾阴阳两虚型患者存在更严重的造血负调控因子紊乱。
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| 关 键 词： | 慢性再生障碍性贫血 microRNA 淋巴细胞亚群 细胞因子 中医证型 |
| 收稿时间： | 2017/5/9 0:00:00 |
Study on Correlation between TCM Syndromes and Peripheral Blood MicroRNA,Lymphocyte Subsets and Cytokines in Patients with Chronic Aplastic Anemia |
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| LI Jun,CHEN Jie,SUN Xue-mei,ZHU Xue-jun,JIANG Peng-jun,CHEN Jian-yi,NI Hai-wen,ZHANG Wen-xi and XIA Wen.Study on Correlation between TCM Syndromes and Peripheral Blood MicroRNA,Lymphocyte Subsets and Cytokines in Patients with Chronic Aplastic Anemia[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2017,31(5):16-21. |
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| Authors: | LI Jun CHEN Jie SUN Xue-mei ZHU Xue-jun JIANG Peng-jun CHEN Jian-yi NI Hai-wen ZHANG Wen-xi and XIA Wen |
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| Institution: | Department of Hematology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029,Department of Pathology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029,Department of Hematology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029,Department of Hematology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029,Department of Hematology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029,Department of Hematology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029,Department of Hematology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029,Department of Hematology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029 and Department of Hematology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029 |
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| Abstract: | |
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| Keywords: | chronic aplastic anemia microRNA lymphocyte subsets cytokines TCM syndromes |
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