肝纤愈对免疫性肝纤维化大鼠TNF-α、IL-1水平的影响

目的建立大鼠免疫损伤性肝纤维化的实验模型,研究中药复方肝纤愈对细胞因子TNF-α、IL-1含量的影响,探讨其抗肝纤维化的作用机制。方法在以人血白蛋白(HSA)诱导的免疫损伤性肝纤维化大鼠模型基础上将肝纤愈分为小剂量(6.1 g/kg)组,大剂量(12.2 g/kg)组灌胃给药,连续8周,以鳖甲软肝片和秋水仙碱为阳性对照组;以放射性免疫方法测定大鼠血清中TNF-α、IL-1含量。结果肝纤愈大剂量组可明显降低HSA诱导免疫性肝纤维化大鼠模型血清中TNF-α、IL-1的水平。结论认为肝纤愈的作用系对TNF-αI、L-1等细胞因子的影响而产生抗肝纤维化作用。

Effect of GanXianYu Compound on the Level of the TNF-α,IL-1 of Immunity Liver Fibrosis in Rats

Objective To explore mechanism of anti-fibrosis,the rat model with immunity liver fibrosis was established to study the effect of compound herbal medicines of GanXianYu on the levels of TNF-α、IL-1.Methods The rat models with immunity liver fibrosis were induced by the human serum albumin,GanXianYu were taken by gastric infusion respectively as little and large dosage group(6.1g.kg~(-1)and 12.2g.kg~(-1)) taken consecutive for 8 weeks.Biejia Ruangan Tablet or colchicines were taken as positive contrast drug.The levels of TNF-α、IL-1 were detected by radioimmunoassay.Results GanXianYu could depress the serum levels of l TNF-α,IL-1 in rat models with immunity liver fibrosis induced by the human serum albumin in large dosage groups.Conclusion GanXianYu could treat liver fibrosis through affect the lever of TNF-α,IL-1 in liver.