急性早幼粒细胞白血病全反式维甲酸治疗时IL—8及其受体表达 …

目的：探讨白细胞介素８（ＩＬ－８）及其Ａ型受体（ＩＬ－８ＲＡ）的表达在全反式维甲酸（ＡＴＲＡ）诱导治疗急性早幼粒细胞白血病（ＡＰＬ）中的临床意义。方法：动态检测ＡＰＬ患者１８例ＡＴＲＡ治疗中血浆ＩＬ－８水平（ＥＬＩＳＡ法）取３例骨髓单个核细胞（ＭＮＣ）加ＡＴＲＡ（１０＾－６ｍｍｏｌ／Ｌ）体外诱导，流式细胞仪动态检测ＭＮＣ膜Ｌ－８ＲＡ的表达。结果：ＭＮＣ加入ＡＴＲＡ诱导７２小时，上清ＩＬ－８水平明显

Expression of interleukin-8 and its receptor in acute promyelocytic leukemia under all-trans retinoic acid treatment]

OBJECTIVE: To evaluate the clinical significance of expression of interleukin-(IL-8) and its type A receptor(IL-8RA) in acute promyelocytic leukemia(APL) patients under all trans-retinoic acid(ATRA) induction. METHODS: Serum IL-8 level of 18 APL patients were dynamically studied(ELISA). Fresh APL cells from 3 patients were cultured with ATRA(10(-6) mmol/L). Supernatant IL-8 level and IL-8RA expression on APL cells were measured by FACS. RESULTS: In vitro, IL-8 concentrations decreased 72 hours after incubation, while IL-8RA increased. In vivo, IL-8 increased more rapidly and markedly than temperature and WBC counts did before retinoic acid syndrome(RA-S) occurred. Serum IL-6 and IL-8 levels significantly increased when the patients suffered infection, and IL-8 increased even before fever. Both IL-8 and D-dimer increased while DIC progressed. CONCLUSION: ATRA inhibited IL-8 secretion of APL cells while increased the expression of IL-8RA. Monitoring serum IL-8 concentrations could predict the development of RA-S and infection. Increase of both IL-8 and D-dimer concentrations suggested DIC progression.