| 高效液相色谱法测定茶新那敏片中盐酸溴己新含量 |
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| 引用本文： | 陈乃江. 高效液相色谱法测定茶新那敏片中盐酸溴己新含量[J]. 中国药业, 2009, 18(8): 35-36 |
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| 作者姓名： | 陈乃江 |
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| 作者单位： | 江苏省连云港市药品检验所,江苏,连云港,222006 |
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| 摘 要： | 目的建立测定茶新那敏片中盐酸溴己新含量的高效液相色谱（HPLC）法。方法色谱柱为Phenomenex C18柱（250inn×4．6mm，5μm），以用三乙胺调节pH至3．5的甲醇-0．2％磷酸溶液（65：35）为流动相，柱温为室温，流速为1．0mL／min，检测波长248nm。结果盐酸溴己新进样量在0．604～2．416μg范围内与峰面积线性关系良好，r=0．9999（n=5），平均回收率为99．45％，RSD为0．82％（n=6）。结论HPLC法简便、准确，可作为茶新那敏片质量控制方法。
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| 关 键 词： | 高效液相色谱法 茶新那敏片 盐酸溴己新 含量测定 |
Content Determination of Bromhexine Hydrochloride In Aminophylline Bromhexine and Chlorphenamine Tablets by HPLC |
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| Chen Naijiang. Content Determination of Bromhexine Hydrochloride In Aminophylline Bromhexine and Chlorphenamine Tablets by HPLC[J]. China Pharmaceuticals, 2009, 18(8): 35-36 |
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| Authors: | Chen Naijiang |
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| Affiliation: | Chen Naijiang (Lianyungang Institute for Drug Control, Lianyungang, Jiangsu, China 222006) |
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| Abstract: | Objective To establish a HPLC method for the content determination of bromhexine hydroehloride in Aminophylline Bromhexine and Chlorphenamine Tablets. Methods The determination was performed on Phenomenex C18 column（250 mm ×4.6 mm,5 μm）with the mobile phase consisted of methanol-0. 2% phosphoric acid solution （65：35, pH was adjusted to 3.5 with triethylamine） at ambient temperature. The flow rate was 1.0 mL/min. The detection wavelength was 248 nm. Results The method showed a good linear relationship for bromhexine hydroehloride within the range of 0. 604 -2. 416 μg （ r =0. 9999, n =5）. The mean recovery rate was 99.45% with RSD was 0. 82% （ n =6）. Conclusion The method is simple, accurate. It can be used for quality control of Aminophylline Bromhexine and Chlorphenamine Tablets. |
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| Keywords: | HPLC Aminophylline Bromhexine and Chlorphenamine Tablets bromhexine hydrochloride content determination |
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