远隔缺血适应渐进模式对大鼠心肌缺血再灌注损伤的研究

目的：分析远隔缺血时不同适应渐进模式对大鼠心肌缺血再灌注损伤的心肌保护作用及其机制。方法：采用大鼠在体心肌缺血-再灌注损伤模型,并应用随机抽签法将60只实验大鼠随机分为假手术组、缺血/再灌注对照组、标准机械后适应组、标准远隔时适应组、正向渐进模式远隔时适应组、负向渐进模式远隔时适应组6组,每组各10只。同时采用称重法检测实验大鼠心肌梗死范围,TUNEL法检测细胞凋亡,分光光度计测MPTP开放程度,并应用蛋白印迹分析检测心肌细胞内p-STAT-3、p-Jak2、p-Akt、p-ERK1/2的蛋白表达情况,比较各组实验结果。结果：与缺血/再灌注对照组相比,标准机械后适应组、标准远隔时适应组、正向渐进模式远隔时适应组、负向渐进模式远隔时适应组的心肌梗死面积、心肌凋亡率以及MPTP开放程度明显更低,且正向渐进模式远隔时适应组显著低于其他组(P<0.05)。蛋白印迹检测显示,正向渐进模式远隔时适应组的p-STAT-3、p-Jak2、p-Akt、p-ERK1/2表达较其他组别显著提高(P<0.05)。结论：正向渐进远隔时适应模式可以发挥较传统标准模式更好的心肌保护作用,其作用机制可能与激活RISK和SAFE途径,抑制MPTP开放有关。

Effects of remote ischemic adaptation progressive model on myocardial ischemia-reperfusion injury in rats

Objective: To analyze the myocardial protective effects and mechanism of different adaptive progressive models on myocardial ischemia-reperfusion injury in rats. Methods: In vivo myocardial ischemia-reperfusion injury model was used, and 60 experimental rats were randomly divided into 6 groups by random lot: sham operation group, ischemia / reperfusion control group, standard mechanical post adaptation group, standard distant time adaptation group, positive progressive mode distant time adaptation group and negative progressive mode distant time adaptation group, with 10 rats in each group. The myocardial infarction area of experimental rats was detected by weighing method, cell apoptosis was detected by TUNEL method, the opening degree of MPTP was measured by spectrophotometer, and the protein expression of p-STAT-3,p-Jak2,p-Akt,p-ERK1/2 in cardiomyocytes was detected by Western blot analysis. The experimental results of each group were compared. Results: Compared with the ischemia/reperfusion control group, the myocardial infarction area, myocardial apoptosis rate and MPTP opening degree of the standard mechanical post adaptation group, the standard remote adaptation group, the positive progressive mode remote adaptation group and the negative progressive mode remote adaptation group were significantly lower, and the positive progressive mode remote adaptation group was significantly lower than other groups (P<0.05). Western blot showed that the expressions of p-STAT-3, p-Jak2, p-Akt and p-ERK1/2 in the adaptation group were significantly higher than those in other groups (P<0.05).Conclusion: The positive progressive distant adaptation model can play a better myocardial protection than the traditional standard model. Its mechanism may be related to activating risk and safe pathways and inhibiting MPTP opening.