三氯乙烯急性染毒对大鼠肝脂质过氧化的影响

用抗氧化剂维生素E预处理后,观察三氯乙烯(3000mg/kg B.W.)一次性经口染毒24小时大鼠肝脏的超氧化物歧化酶、谷胱甘肽过氧化物酶等抗氧化酶活力及丙二醛含量的变化.结果表明,三氯乙烯染毒组肝脏中丙二醛含量、超氧化物歧化酶活力及血清谷丙转氨酶、谷草转氨酶活力均高于对照组(P<0.01),同时,肝细胞出现脂肪变与坏死;而维生素E干预组的丙二醛含量、超氧化物歧化酶活力及血清谷丙转氨酶、谷草转氨酶活力均分别低于三氯乙烯染毒组(P<0.01),肝细胞脂肪变与坏死程度有所改善,说明三氯乙烯急性染毒可引起肝脏脂质过氧化反应及肝损害,肝脏超氧化物歧化酶活力升高可能是机体受自由基及脂质过氧化反应刺激而诱导产生的一种适应性反应,维生素E对三氯乙烯所致的肝损害有一定的保护作用.

Effect of Trichloroethylene on Lipid Peroxidation in the Liver of Rats As acute Exposure

After single oral administration(by gavage)of trichloroethylee (3000mg/kg B.W.),the serum and liver were collected.The activity of serum GPT and GOT(as indices of liver damage)were measured.Also,The activity of superoxide dismutase(SOD)glutathione peroxidase(GSH-Px)and the level of malondialdehy de (MDA)were analysed in the liver homogenate of the rats.The results revealed the activity of liver SOD and serum GPT、GOT in the rats acutely exposed to trichlorothy lene(TCE)was sinificantly higher than that of those the control(P<0.01).pretreatment with vitamin E.could reduce the level of liver MDA and the acitivity of liver SOD、seum GPT and GOT.It is suggested that TCE might induce lipid peroxidation in the live of rats and vitamine E could protect liver from TCE~induced liver damage in some degree.