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Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry
Authors:Apurva S. More  Ronald T. Toth  Solomon Z. Okbazghi  C. Russell Middaugh  Sangeeta B. Joshi  Thomas J. Tolbert  David B. Volkin  David D. Weis
Affiliation:1. Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas 66047;2. Macromolecule and Vaccine Stabilization Center, University of Kansas, Lawrence, Kansas 66047;3. Department of Chemistry, University of Kansas, Lawrence, Kansas 66045
Abstract:
We have used hydrogen exchange–mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from Pichia pastoris, 2 of which were prepared using subsequent in vitro enzymatic treatments. Progressively decreasing the size of the N-linked N297 oligosaccharide from high mannose (Man8-Man12), to Man5, to GlcNAc, to nonglycosylated N297Q resulted in progressive increases in backbone flexibility. Comparison of these results with recently published physicochemical stability and Fcγ receptor binding data with the same set of glycoproteins provide improved insights into correlations between glycan structure and these pharmaceutical properties. Flexibility significantly increased upon glycan truncation in 2 potential aggregation-prone regions. In addition, a correlation was established between increased local backbone flexibility and increased deamidation at asparagine 315. Interestingly, the opposite trend was observed for oxidation of tryptophan 277 where faster oxidation correlated with decreased local backbone flexibility. Finally, a trend of increasing C'E glycopeptide loop flexibility with decreasing glycan size was observed that correlates with their FcγRIIIa receptor binding properties. These well-defined IgG1-Fc glycoforms serve as a useful model system to identify physicochemical stability and local backbone flexibility data sets potentially discriminating between various IgG glycoforms for potential applicability to future comparability or biosimilarity assessments.
Keywords:stability  antibody  hydrogen exchange  mass spectrometry  glycosylation
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