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| 全反式维甲酸诱导分化脑胶质瘤干细胞治疗胶质瘤的实验研究 | |
| 引用本文： | 高 歌 董永飞 李明武等. 全反式维甲酸诱导分化脑胶质瘤干细胞治疗胶质瘤的实验研究[J]. 吉林医学, 2014, 0(14): 5-6 |
| 作者姓名： | 高 歌 董永飞 李明武等 |
| 作者单位： | 安徽医科大学附属省立医院,安徽合肥230001 |
| 基金项目： | 安徽医科大学校科学研究[基金编号：2010xkj089]; 安徽省自然科学基金青年项目[基金编号：1208085QH141] |
| 摘 要： | 目的:探讨全反式维甲酸对大鼠C6脑胶质瘤细胞增殖的抑制作用及其机制。方法:建立大鼠C6脑胶质瘤模型,腹腔注射全反式维甲酸后,采用MRI检测胶质瘤的体积变化、流式细胞仪检测细胞增殖时相变化及免疫组化检测p27Kipl蛋白的变化。结果:全反式维甲酸组较对照组肿瘤体积明显减小(P<0.05);Go期细胞比例明显增加(P<0.05),S期细胞比例明显减少(P<0.05);P27KiPl蛋白的表达明显增加(P<0.05)。结论:全反式维甲酸通过上调p27Kipl蛋白的表达而抑制大鼠C6脑胶质瘤细胞增殖。 |
| 关 键 词： | 全反式维甲酸 脑胶质瘤干细胞 胶质瘤 p27Kipl蛋白 |
Experimental study of differenti therapy by all-trans retinotic acid on glioma stem cells for glioma |
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| Affiliation: | GAO Ge,DONG Yong -fei,Ll Ming -wu,et al ( Anhui Provincial Hospital,Hefei Anhui 230001 ,China) |
| Abstract: | Objective To study the cell prolifation inhibition and mechanism of all-trans retinotic acid on C6cells of rats. Methods The model of C6glioma cells on rats was made. After intraperitoneal injection of all-trans retinotic acid,the change of volume was detected by MRI,the phase change of cell proliferation was detected by FCM,the change of p27Kipl was detected by IHC. Results In all-trans retinotic acid group,the tumor volume reduced significantly（ P〈0. 05）,the ratio of Go-phase cell increased significantly（ P〈0. 05）,the ratio of S-phase cell reduced significantly（ P〈0. 05）,the expression of p27Kipl increased significantly（ P〈0. 05）. Conclusion All-trans retinotic acid inhibits the cell multiplication of C6cells by increasing the expression of p27Kipl. |
| Keywords: | All-trans retinotic acid Glioma stem cells Glioma p27Kipl |
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