c-kit与p27蛋白在胃肠道间质瘤诊治中的临床意义

目的研究c-kit和p27蛋白在胃肠道间质瘤中的表达及其与间质瘤临床病理特征的关系,为GIST诊断、预后评估提供客观指标。方法收集54例手术切除的胃肠道间质瘤标本,同时取距瘤灶>5cm的正常组织,应用免疫组织化学S-P检测肿瘤组织及正常组织中c-kit和p27蛋白的表达。结果54例胃肠道间质瘤中,c-kit和p27蛋白阳性表达率分别为94.44%和59.26%。c-kit蛋白表达水平在GIST不同分化程度、有无复发及远处转移的差异无显著性意义(P>0.05),p27蛋白表达水平在GIST不同分化程度、有无局部复发间差异有显著性(P<0.01)。结论c-kit作为特异敏感的标志物,对GIST的诊断及鉴别诊断有重要意义,而检测p27蛋白可作为评估GIST恶性程度和判断预后的重要指标。

Expression and clinical significance of p27 protein and c-kit protein in gastrointestinal stromal tumors

Objective To investigate the expression of c kit and p27 protein in gastrointestinal stromal tumors(GIST), and discuss the relationship between their expression and clinicopathological characteristics. Methods Immunohistochemical staining was used to detect the expression of c kit and p27 in 54 cases of GIST and corresponding normal tissues. Results In 54 cases GIST, the expression of c kit and p27 protein were 94.44 % and 59.26 % respectively. The expression level of c kit was not found to be correlated with the degree of differentiation, tumor recurrence and distant metastasis(P >0.05). The low level expression of p27 protein was inversely correlated with degree of differentiation and tumor recurrence of GIST(P <0.01). Conclusions These data shows that the expression of c kit is an important factor for diagnosis and differential diagnosis of GIST, and p27 expression is related to the progression of GIST.