| 西藏冬虫夏草水提液HPLC指纹图谱研究及核苷类成分测定 |
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| 引用本文： | 高晓霞,周伟平,谢明容,代安国,熊卫萍,章卫民.西藏冬虫夏草水提液HPLC指纹图谱研究及核苷类成分测定[J].广东医药学院学报,2014(1):44-51. |
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| 作者姓名： | 高晓霞 周伟平 谢明容 代安国 熊卫萍 章卫民 |
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| 作者单位： | [1]广东药学院药科学院,广东广州510006 [2]西藏农牧科学院蔬菜研究所,西藏拉萨850000 [3]广东省微生物研究所,广东广州510070 |
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| 基金项目： | 国家科技支撑计划项目（2007BA132802）;国家自然科学基金项目（30870450） |
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| 摘 要： | 目的建立西藏不同产区27批冬虫夏草水提液的高效液相色谱指纹图谱，并定量测定其6种主要核苷类成分（尿苷、肌苷、鸟苷、胸苷、腺苷、2＇-脱氧腺苷）的质量分数。方法采用高效液相色谱法，色谱柱为Agilent Eclipse XDB—C18柱（250mm×4．6mm，5μm），检测波长260nm，以甲醇．水为流动相进行梯度洗脱，柱温25℃，流速O．8mL·min^-1。结果建立了冬虫夏草水提液的HPLC指纹图谱，包含11个共有峰，各峰分离良好，指认出尿苷、肌苷、鸟苷、胸苷、腺苷、2’-脱氧腺苷6个特征成分．同时测定了其质量分数；6种特征成分质量浓度分别在10．71-96．4（r=0．9999），8．50～76．41（r：0．9999）。7．838-70．54（r=0．9999），1．120-10．08（r=0．9998），5．715-51．44（1-=0．9999），0．2625～2．363μg·mL^-1（r=0．9997）范围内与峰面积呈良好的线性关系；平均回收率依次为96．5％、97．7％、97．5％、95．2％、96．8％、101．5％，RSD分别为2．3％、2．7％、2．0％、2．6％、3．0％、2．7％（n=6）。结论本试验建立的指纹图谱可同时对冬虫夏草的6种主要核苷类成分进行分析，对评价藏产冬虫夏草的质量具有重要意义。
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| 关 键 词： | 冬虫夏草 HPLC 指纹图谱 核苷类成分 |
HPLC fingerprint of aqueous extract of Ophiocordyceps sinensis from Tibet and determination of nucleosides components |
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| Authors: | GAO Xiaoxia ZHOU Weiping XIE Mingrong DAI Anguo XIONG Weiping ZHANG Weimin |
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| Institution: | 1. School of pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; 2. Institute of Vegetable Sciences, Tibet Academy of Agricultural and Animal Husbandry Sciences ,Lasa 850000, China; 3. Guangdong Institute of Microbiology, Guangzhou 510070, China) |
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| Abstract: | Objective To establishan HPLC fingerprint of aqueous extract of Ophiocordyceps sinensis from the main producing area of Tibet, and explore a method to determine 6 nucleosides components （ uridine, inosine, guanosine, thymidine, adenosine and 2＇-deoxyadenosine）. Methods The HPLC analysis was performed on Agilent Eclipse XDB-C18 column （250 mm × 4.6 mm,5 μm） and detected at 260 nm. The mobile phase was consisted of methanol and water using a gradient elution. The column temperature was 25 ℃ and the flow rate was 0.8 mL · min^-1. Results The HPLC fingerprint was established, and eleven common characteristic peaks had a satisfactory peak separation. 6 components, including uridine, inosine, guanosine,thymidine, adenosine and 2＇-deoxyadenosine, were identified and determined. The linearity of the 6 components were in 10.71-96.4（r= 0.999 9） ,8.50-76.41 （r=0.999 9） ,7.838-70.54（r=0. 999 9）, 1.120-10.08（r= 0.999 8）,5.715-51.44（r=0.999 9）,0.262 5-2.363 μg · mL^-1（r= 0.999 7）, respectively. The average recovery were 96. 5%, 97.7%, 97.5%, 95.2%, 96.8% and 101.5%, and the RSD were 2. 3%, 2. 7%, 2.0%, 2. 6%, 3.0% and 2.7% （ n = 6）. respectively. Conclusion The HPLC fingerprint was established, and it provides a method for the simultaneous analysis of 6 main nucleosides components in 0. sinensis,which is important for the quality evaluation of 0. sinensis from Tibet. |
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| Keywords: | Ophiocordyceps sinensis HPLC fingerprint nucleosides components |
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