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| 高效液相色谱法测定叶酸片中叶酸含量 | |
| 引用本文： | 高桂花,盛筱,李雪冰. 高效液相色谱法测定叶酸片中叶酸含量[J]. 济宁医学院学报, 2010, 33(4): 238-240. DOI: 10.3969/j.issn.1000-9760.2010.04.004 |
| 作者姓名： | 高桂花 盛筱 李雪冰 |
| 作者单位： | 济宁医学院药学院,山东,日照,276826;济宁医学院药学院,山东,日照,276826;济宁医学院药学院,山东,日照,276826 |
| 摘 要： | 目的建立高效液相色谱法测定叶酸片中叶酸含量的方法。方法采用Diamonsil C18色谱柱（250mm×4.6mm,5μm）,以甲醇-水-冰乙酸（40：60：0.1）为流动相,流速为1.0ml/min,检测波长为254nm。结果叶酸在20.08～180.72μg/ml范围内有良好的线性关系（r=0.9999）,样品平均加样回收率为100.5%,RSD为1.2%。结论本方法快速,可靠,有良好的精密度和准确度,适应于叶酸片的质量控制。 |
| 关 键 词： | 高效液相色谱法 叶酸 含量测定 |
Determination of Folic Acid in Folic Acid Tablets by HPLC |
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| GAO Gui-hua,SHENG Xiao,LI Xue-bing. Determination of Folic Acid in Folic Acid Tablets by HPLC[J]. Jounal of Jinning Medical College, 2010, 33(4): 238-240. DOI: 10.3969/j.issn.1000-9760.2010.04.004 | |
| Authors: | GAO Gui-hua SHENG Xiao LI Xue-bing |
| Affiliation: | (Pharmaceutical College,Jining Medical University,Rizhao 276826,China) |
| Abstract: | Objective To establish an HPLC method for the determination of Folic Acid in Folic Acid Tablets. Methods Folic Acid was determined on HPLC instrument fixed a Diamonsil C18 column（250 mm×4.6 mm,5 μm）with UV detection at 254nm.The mobile phase was water（containing 0.1%glacial acetic acid）-methanol （60：40）with the flow rate at 1.0 ml/min.Results The liner range of Folic Acid was 20.08μg/ml-180.72μg/ml （r=0.9999）,and the average recovery was 100.5% with RSD1.2%.Conclusion The method is simple,sensitive,accurate and can be used for the quality control of Folic Acid Tables. |
| Keywords: | HPLC Folic Acid Content determination |
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