吴茱萸中4种单体成分致肾细胞毒性的初步研究

目的探讨吴茱萸中成分吴茱萸碱、吴茱萸次碱、吴茱萸内酯和辛弗林在体外对人胚肾细胞(HEK-293)的影响。方法采用MTT法检测吴茱萸碱、吴茱萸次碱、吴茱萸内酯和辛弗林对肾细胞活力的影响;给药后检测肾细胞培养上清液中的功能性指标乳酸脱氢酶(LDH)含量;采用倒置相差显微镜对给药后的细胞形态进行观察。结果 MTT法显示,8.3～33.2 g.mL-1的吴茱萸碱、5～40 g.mL-1的吴茱萸次碱和50～200 g.mL-1的吴茱萸内酯对HEK-293细胞活力有明显的抑制作用(P<0.01 or 0.05),辛弗林对肾细胞活力无明显影响。4.15～33.2 g.mL-1的吴茱萸碱、5～20 g.mL-1的吴茱萸次碱和50～200 g.mL-1的吴茱萸内酯能显著升高肾细胞上清液中的LDH(P<0.01),给予辛弗林后LDH无变化。给予4.15～33.2 g.mL-1的吴茱萸碱和100～200 g.mL-1的吴茱萸内酯后,肾细胞均不同程度的皱缩、减少、甚至死亡,吴茱萸次碱和辛弗林对肾细胞形态无明显影响。结论吴茱萸碱、吴茱萸次碱和吴茱萸内酯对肾细胞均有毒性作用,而辛弗林对肾细胞无毒性作用。

Preliminary Study on Nephrocytes Toxicity Induced by Four Traditional Chinese Medicine Monomers in Evodia Rutaecarpa

Objective To test the toxic effects of evodiamine, rutaecarpine, evodin and synephrine on human embryonic kidney 293 cells（HEK293）. Methods MTT assay was used to test the cell viability of evodiamine, rutaecarpine, evodin and synephrine on nephrocytes. The contents of LDH in nephrocytes supernatant were detected.After given the four monomers, morphological changes of nephrocytes were observed by inverted phase contrast microscope. Results The MTT assay showed that 8.3-33.2μg·-1 evodiamine, 5-40 μg·mL-1 rutaecarpine and 50-200μg·mL-1 evodin could inhibit HEK-293 cells viability obviously（P 〈0.01 or P 〈0.05）, while synephrine had no inhibition on HEK-293 cells viability. 8.3-33.2μg·mL-1 evodiamine, 5-20μg·mL-1 rutaecarpine and 50-200 μg·mL-1 evodin could increase the contents of LDH in nephrocytes supernatant（P〈0.01 or P〈0.05）, while synephrine couldn＇t. Cell morphology showed that evodiamine, evodin could damage the shapes ofnephrocytes and cells mortality raised as the increase of their concentration. On the other side, evodiamine and synephrine had no influence. Conclusion Evodiamine, rutaecarpine and evodin may cause nephrocytes toxicity while synephrine could not.