癌胚抗原相关细胞粘附因子19与乳腺癌相关基因关系

目的 研究癌胚抗原相关粘附因子19(CEACAM19)mRNA表达水平与乳腺癌患者雌激素受体(estrogen receptor,ER)、孕激素受体(progestrone receptor,PR)、Her-2(human Epidermal growth factor receptor type 2)及CA15-3(carbohydrate atigen 15-3)表达的关系及临床意义。方法 应用实时荧光定量聚合酶链式反应(real-time RT-PCR)方法检测52例乳腺原发癌和配对淋巴转移癌以及130例乳腺原发癌的CEACAM19 mRNA的表达水平,比较52例乳腺原发癌和配对淋巴转移癌以及130例乳腺原发癌的CEACAM19 mRNA的表达水平,分析CEACAM19 mRNA表达水平与ER、PR及Her-2表达的关系。结果 52例乳腺癌淋巴结转移癌CEACAM19 mRNA表达水平(0.788±0.87)明显高于原发癌(0.431±0.381)(t=-3.289,P<0.05)。130例患者中,115例有雌激素受体检测结果,其中雌激素受体阳性组80例,CEACAM19 mRNA高表达者34例(34/80,42.5%);雌激素受体阴性组35例,CEACAM19 mRNA高表达24例(24/35,68.6%),2组CEACAM19 mRNA高表达占比差异有统计学意义(P<0.05)。CEACAM19 mRNA表达水平与孕激素受体、Her-2及CA15-3表达无相关性。结论 CEACAM19 mRNA的表达水平与乳腺癌患者雌激素受体表达水平有关。

Clinical significance and relationship of CEACAM19 mRNA expression with ER,PR and Her-2 in breast cancer patients

Objective To study the clinical significance of carcino-embryonic antigen related cellular adhesion molecule 19(CEACAM19)mRNA and the relationship between the CEACAM19 mRNA expression,estrogen receptor(ER),progestrone receptor(PR),human epidermal growth factor receptor type 2(Her-2),and carbohydrate atigen 15-3(CA15-3) among breast cancer patients.Methods Totally 130 newly pathologically diagnosed breast cancer patients aged 32-70 years were recruited at a hospital from October 2008 to October 2014;52 metastatic lymph node and 130 primary breast cancer tissue specimens were collected from the patients for the detection of CEACAM19 mRNA expression with real-time PCR and the expressions of ER,PR,Her-2,CA15-3 were also measured simultaneously for most of the patients.Results The average expression of CEACAM19 mRNA in 52 metastatic lymph nodes was significantly higher than that in primary breast cancer tissues(0.788±0.87 vs.0.431±0.381,t=-3.289;P<0.05).Of the 115 patients with ER detection,80 were ER-positive;the ER-positive patients had a significantly lower proportion of high CEACAM19 mRNA expression(42.5%,34/80) compared to that of the ER-negative patients(68.6%,24/35)(P<0.05).No significant correlations were observed between the expression of CEACAM19 mRNA and detection results of PR,Her-2,and CA15-3.Conclusion CEACAM19 mRNA expression correlates with ER expression among breast cancer patients.