鲍曼不动杆菌ATCC19606多重耐性分析及耐药基因的克隆

鲍曼不动杆菌已成为重要的院内感染病菌。我们测定了鲍曼不动杆菌ATCC19606的药物最小抑制浓度（MIC），结果显示该菌株对多种抗菌药物都有很高的耐性，如链霉素、诺氟沙星、氯霉素、红霉素、四环索、氨苄西林及一些其他的抗菌染剂。利用大肠埃希菌超敏菌株KAM32作为宿主，从鲍曼不动杆菌ATCC19606染色体DNA中克隆耐药基因，共获得9个使宿主细胞产生耐药性的杂合质粒，其中1个为单一耐药，其余全部为多重耐药。根据药物特异性分析可知，具有不同耐药图谱的杂合质粒携带不同类型的耐药基因。由此揭示鲍曼不动杆菌ATCC19606的多重耐药有多种机制参与。

Multiple resistance in Acinetobacter baumannii ATCC 19606 and cloning of genes responsible for the resistance

Drug resistance of Acinetobacter baumannii ATCC 19606 was tested in this study. The result showed fairly high resistance to many antimicrobial agents tested including streptomycin, norfloxacin, chloramphenicol, erythromycin, tetracycline, ampicillin, and antimicrobial dyes. Using the drug-hypersensitive strain of Escherichia coli KAM32 as the host, we cloned the genes responsible for multiple resistance from chromosomal DNA of A. baumannii ATCC 19606. We obtained 9 hybrid plasmids that made host cells resistant to several antimicrobial agents. Many of the transformants harboring each of the plasmids showed multiple resistance, and one showed resistance to specific drug. The hybrid plasmids were classified into several groups based on their drug specificity. It appears that each class of plasmid carries different types of drug resistance genes. Analysis of such genes will reveal the various mechanisms involved in multiple resistance in A. baurnannii ATCC 19606.