骨质疏松大鼠的骨形成蛋白-4诱导成骨信号转导机制变化

目的 观察骨形成蛋白4(BMP-4)及其信号转导蛋白(Smad)5、6在去卵巢大鼠骨组织中的表达.方法 采用切除大鼠双侧卵巢法复制骨质疏松模型,术后94 d,用双能X线骨密度仪检测骨密度;用Western印迹法检测骨组织中BMP-4和Smad5、6蛋白表达水平.结果 与正常组比较,模型空白组大鼠股骨骨密度显著下降(P＜0.01);BMP-4和Smad5蛋白表达水平显著降低(P＜0.01),Smad6蛋白表达水平明显升高(P＜0.01).结论 骨组织中BMP-4、Smad5蛋白表达水平的下降和Smad6蛋白表达水平的升高,可能是骨质疏松发生的重要机制之一.

Signal transduction mechanism change of bone morphogenetic protein-4(BMP-4) in osteoporosis

Objective To study the expression and significance of the bone morphogenetic protein-4 (BMP-4) and signal transduction protein Smad5, 6 in the bone tissue of ovariectomized rat. Methods The osteoporosis (OP) model rat was established by removal bilateral ovaries. Bone mineral density (BMD) was measured by Dual-Energy X Ray, BMP-4, Smad5, 6 protein were measured by Western blotting on 94 days after surgery. Results Compared with the normal rats, the BMD of femoral bone in vitro in model group decreased obviously (P＜0.01), the expressions of BMP-4 and Smad5 protein decreased obviously(P＜0.01) and the expression of Smad6 protein increased obviously(P＜0.01) in model group. Conclusions Conclusions The decrease of BMP-4 and Smad5 and the increase of Smad6 in bone tissue may be one of the mechanisms of postmenopausal OP.