骨诱导活性材料复合富血小板纤维蛋白在拔牙位点引导新骨形成的骨计量学研究

目的：研究骨诱导活性材料（Osteoinduction Active Material，OAM）复合富血小板纤维蛋白（Platelet－rich Fibrin，PRF）在拔牙位点引导新骨形成的作用，考察OAM复合PRF促进拔牙位点保存修复的效果。方法：选取12月龄健康雄性Beagle犬6只，建立动物模型，微创拔除两侧上、下颌第二侧切牙。4个位点随机分为A、B、C、D 4组，分别植入PRF与OAM复合物、单一的PRF或OAM，以及空白对照。术后1个月和3个月，分别处死3只动物并立即在拔牙位点部位取材，制作组织切片H．E和甲苯胺蓝染色，进行骨计量学分析。结果：A组骨小梁宽度、位点区骨量和成骨细胞数均明显大于其他3组（P＜0．01）。析因分析显示OAM复合PRF在拔牙位点区引导新骨形成的效果优于单独使用OAM或PRF（P＜0．01）。结论：OAM复合PRF可以增加拔牙位点区的成骨细胞数。骨量明显增加，有助于骨改建和改善拔牙位点区牙槽骨质量。

Osteoinductive active material complex with Platelet-rich fibrin guide the new bone formation on extraction sites by bone histomorphometry

Objective:The aim of the study was to explore study the effect that osteoinductive active material(OAM) combined with platelet-rich fibrin (PRF) lead to the formation of new bone in the extraction site,and to investigate the re-sult that the composite promoted the preservation and restoration of extraction site. Method:The 12-month-old male Beagle dogs with the number of six were extracted both second maxillary and mandibular lateral incisors with the minimally extract-ed method. To establish the animal model,the four sites were randomly divided into four groups,as A,B,C and D. The for-mer three groups were filled with OAM-PRF composite,OAM and PRF respectively,and D controlled group. Three dogs were randomly sacrificed after one-month treatment,and the left three dogs were sacrificed after three-month treatment. The samples were taken from the alveolar bone and as followed stained with toluidine blue and H.E respectively for histomorpho-metric observation. Result:The trabecular width,trabeculae area and an osteoblastic number of group A are significantly higher than the other three groups(P<0.01). The factorial analysis shows the effect of the OAM-PRF complex is superior to use OAM or PRF alone,guiding the new bone formation in the extraction site area. Conclusion:The number of osteoblasts can increase significantly under the effect of OMA complex with PRF,which contribute to bone remodeling and improving the quality of alveolar bone in tooth extraction site.