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| HPLC法测定板蓝根滴丸中腺苷的含量 | |
| 引用本文： | 符敬伟,黄志军,刘树春,张庆伟,王润玲. HPLC法测定板蓝根滴丸中腺苷的含量[J]. 中国药房, 2008, 19(33): 2607-2608 |
| 作者姓名： | 符敬伟 黄志军 刘树春 张庆伟 王润玲 |
| 作者单位： | 天津医科大学药学院，天津市长征医院，天津市食品药品监督管理局 |
| 摘 要： | 目的:建立以高效液相色谱(HPLC)法测定板蓝根滴丸中腺苷含量的方法。方法:色谱柱为Phenomenex-C18(250mm×4.6mm,5μm),流动相为乙腈-水(6∶94),流速为1mL.min-1,检测波长为260nm。结果:腺苷的检测浓度在6.36～31.8μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9995);平均回收率为104.93%,RSD=0.94%(n=6)。结论:本方法准确、快速、简便,可用于板蓝根滴丸的质量控制。 |
| 关 键 词： | 板蓝根滴丸 腺苷 高效液相色谱法 含量测定 |
Determination of Adenosine in Banlangen Dropping Pills by HPLC |
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| FU Jing-wei,HUANG Zhi-jun,LUI Shu-chun,ZHANG Qing-wei,WANG Run-ling. Determination of Adenosine in Banlangen Dropping Pills by HPLC[J]. China Pharmacy, 2008, 19(33): 2607-2608 | |
| Authors: | FU Jing-wei HUANG Zhi-jun LUI Shu-chun ZHANG Qing-wei WANG Run-ling |
| Affiliation: | FU J ing-wei, ZHANG Qing-wei, WANG Run-ling ,HUANG Zhi-jun, LUI Shu-chun(College of Pharmacy, Tianjin Medical University, Tianjin 300070, China;Tianjin Changzheng Hospital, Tianjin 300120, China;Tianjin Food and Drug Administration, Tianjin 300051, China) |
| Abstract: | OBJECTIVE： To establish an HPLC method for the assay of Adenosine in Banlangen dropping pills. METH- ODS： The sample was determined by HPLC on a Phenomenex - C18 column（250 mm × 4.6 mm, 5μm） .The mobile phase consisted of acetonitrile-water（6 ： 94） at a flow rate of 1 mL · min^-1 and detected at 260 nm.RESULTS： The calibration curve was linear in the range of 6.36-31.8 μg · mL^- 1. The average recovery of sample was 104.93% （RSD = 0.94%, n = 6） . CONCLUSION ：This method is accurate,rapid and simple,and it can be used for the quality control of Banlangen dropping pills. |
| Keywords: | Banlangen dropping pills Adenosine HPLC Content determination |
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