| 低氧、无血清培养对人骨髓间充质干细胞增殖、凋亡的影响 |
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| 引用本文： | 蒋能刚,胥劲,邱琳,唐莉,朱鸿明,李秀群,陈晓禾. 低氧、无血清培养对人骨髓间充质干细胞增殖、凋亡的影响[J]. 山东医药, 2010, 50(11): 1-3 |
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| 作者姓名： | 蒋能刚 胥劲 邱琳 唐莉 朱鸿明 李秀群 陈晓禾 |
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| 作者单位： | 1. 四川大学华西医院,成都,610041
2. 华西医院生物治疗国家重点实验室干细胞与组织工程研究室 |
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| 摘 要： | 目的观察人骨髓间充质干细胞（hBM—MSCs）对低氧环境的适应能力，为其临床应用提供依据。方法体外培养hBM—MSCs，根据培养条件分为四组，常氧正常血清组培养条件为20％O2及10％FBS／HD、常氧无血清组为20％O2及0％FBS／HD，低氧正常血清组为1．5％O2及10％FBS／HD，低氧无血清组为1．5％O2及0％FBS／HD，细胞培养6、12、24、48、72、96h后采用MTT法检测四组增殖状况；流式细胞术检测四组凋亡情况。结果在10％FBS培养下，48h时低氧可显著促进细胞增殖；在整个观察时间内，低氧培养不会促进hBM—MSCs凋亡；同时元血清培养时细胞增殖受抑。结论在正常的血清培养条件下，hBM—MSCs对低氧有较好的耐受性，但是无血清培养不利于hBM—MSCs增殖，hBM-MSCs可作为缺氧相关组织修复的种子细胞。
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| 关 键 词： | 骨髓间充质干细胞 低氧 增殖 凋亡 |
Effects of hypoxia and serum deprivation on the proliferation and apoptosis of human bone marrow derived mesenchymal stem cells |
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| JIANG Neng-gang,XU Jin,QIU Lin,TANG Li,ZHU Hong-ming,LI Xiu-qun,CHEN Xiao-he. Effects of hypoxia and serum deprivation on the proliferation and apoptosis of human bone marrow derived mesenchymal stem cells[J]. Shandong Medical Journal, 2010, 50(11): 1-3 |
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| Authors: | JIANG Neng-gang XU Jin QIU Lin TANG Li ZHU Hong-ming LI Xiu-qun CHEN Xiao-he |
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| Affiliation: | JIANG Neng-gang1,XU Jin,QIU Lin,TANG Li,ZHU Hong-ming,LI Xiu-qun,CHEN Xiao-he(1 Department of Laboratory Medicine,West China Hospital of Sichuan University,Chengdu 610041,P.R.China) |
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| Abstract: | Objective To investigate whether human bone marrow derived mesenchymal stem cells could resist the hypoxia condition in vitro,so as to provide basis for its clinical application.Methods hBM-MSCs were cultured in vitro and divided into four groups according to the culture condition,the normoxia-normal serum group was cultured with 20%O2 and 10%FBS+ DMEM-HG(FBS/HD),the normoxia-serum deprivation group was cultured with 20%O2 and 0%FBS/HD,the hypoxia-normal serum group was cultured with 1.5%O2 and 10%FBS/HD,th... |
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| Keywords: | mesenchymal stem cells hypoxia proliferation apoptosis |
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