激发性CD28单抗对肿瘤患者外周血FOXP3^＋Treg细胞的下调作用及意义

目的探究肿瘤患者外周血单个核细胞（PBMC）中FOXP3^＋Treg经CD28单抗激发后的改变及意义。方法采用腹水诱生法及免疫亲和层析法进行单抗的制备和纯化，Ficoll密度梯度离心法获取外周血PBMC，经激发性CD28单抗10μg／mL共培养后经免疫荧光及流式细胞术分析其中FOXP3^＋Treg的表达以及FOXP3^＋Fas^＋Treg和FOXP3^＋FasL^＋Treg的表达。结果免疫荧光和流式细胞术显示肿瘤患者外周血中FOXP3^＋Treg表达比例为（6．208±2．754）％，而对照组为（2．653±0．638）％。FOXP3^＋FasL^＋Treg细胞经CD28单抗活化后FasL表达上调为（7．252±2．23）％，而健康对照组为（7．96±2．938）％，FOXP3^＋Fas^＋Treg活化前后的比例分别为（1．467±0．590）％和（3．692±1．822）％。结论肿瘤患者外周血中PBMC经CD28单抗激发后FOXP3^＋Treg的比例下调（P〈0．01），提示该抗体能够使肿瘤患者的免疫功能得到一定程度的恢复与提高。

The role and significance of CD28 monoclonal antibody stimulating peripheral blood of patients with tumors of FOXP3+Treg cells

Objective To explore the change and significance of FOXP3^＋ Treg within peripheral blood mononudear cells（PBMC） ,which stimulated by CD28 monoelonal antibody in tumor patients. Methods Ascites induced immune and pro - law chromatography for purification and preparation of the monoclonal antibody, Ficoll density gradient centrifugation to obtain peripheral blood PBMC, and/or stimulated by CD28 monoclonal antibody 10 μg/mL after co -culture by immunofluorescence and flow eytometry analysis of FOXP3^＋ Treg, as well as the expression of FOXP3^＋ Fas^＋ Treg and FOXP3^＋ FasL^＋ Treg. Results Immunofluorescenee and flow eytometry showed that the peripheral blood of tumor patients with FOXP3^＋ Treg expression ratio（6. 208±2.754）% ,while the control group （2.653 ± 0.638 ）%. FOXP3^＋ FasL^＋ Treg cells activated by CD28 monoelonal antibody for the FasL expression（7.252 ± 2.23 ）%, and healthy control group（ 7.96 ± 2.938 ）% Before and after activation the ratio of FOXP3^＋Fas^＋Treg（1.467 ± 0.590）% ,（3.692 ± 1. 822）%. Conclusion The ratio of FOXP3^＋Treg within PBMC in tumor patients decreased（P 〈 0.01 ）after stimulating by CD28 monoclonal antibody which suggested the patients immune function recover and improve.