|
|||||
|
|
| 胶质瘤中Axin基因点突变检测及表达研究 | |
| 引用本文： | 邵秋杰,李青,金利华,赵一岭,张英华,林圣彩. 胶质瘤中Axin基因点突变检测及表达研究[J]. 中华神经外科疾病研究杂志, 2003, 2(4): 334-337 |
| 作者姓名： | 邵秋杰 李青 金利华 赵一岭 张英华 林圣彩 |
| 作者单位： | 1. 第四军医大学西京医院病理科,陕西,西安,710032 2. 厦门大学生命科学学院,福建,厦门,361000 3. 武警8610部队医院内科,辽宁,盘锦,124010 |
| 基金项目： | 高等学校骨干教师计划资助项目，归国留学人员科研启动基金资助项目 (1 999747) |
| 摘 要： | 目的 检测胶质瘤中Axin基因的点突变及其表达情况，初步探讨Axin与胶质瘤发生的关系。方法 采用聚合酶链反应-单链构象多态性(PCR—SSCP)技术及DNA测序方法检测Axin基因外显子8，9及10在28例胶质瘤中的突变情况；同时对上述胶质瘤及正常脑组织进行免疫组化染色。结果 在28例胶质瘤组织中Axin的第10个外显子共有6例样本(21．4％)3处发生了错义突变；3例3处发生了同义突变；28例胶质瘤中8例(28．6％)Axin表达阳性，正常脑组织中神经元表达阳性，神经胶质细胞表达阴性，检测到突变的样本中1例表达阳性。结论 Axin基因的点突变可能参与胶质瘤的发生。 |
| 关 键 词： | 胶质瘤 Axin 基因点突变 检测 基因表达 聚合酶链反应-单链构象多态 DNA测序 |
| 文章编号： | 1671-2897(2003)02-334-04 |
| 修稿时间： | 2003-05-05 |
Detection of point mutations of Axin gene and its expression in gliomas |
|
| SHAO Qiujie ,LI Qing ,JIN Lihua ,ZHAO Yiling ,ZHANG Yinghua ,LIN Shengcai. Detection of point mutations of Axin gene and its expression in gliomas[J]. Chinese Journal of Neurosurgical Disease Research, 2003, 2(4): 334-337 | |
| Authors: | SHAO Qiujie LI Qing JIN Lihua ZHAO Yiling ZHANG Yinghua LIN Shengcai |
| Affiliation: | SHAO Qiujie 1,LI Qing 1,JIN Lihua 2,ZHAO Yiling 1,ZHANG Yinghua 3,LIN Shengcai 2 1Department of Pathology,Fourth Military Medical University,Xi'an 710032, 2School of Life Science,Xiamen University,Xiamen 361000, 3Department of Medicine,Armed Police 8610 Hospital,Panjin 124010,China |
| Abstract: | Objective To detect the point mutations of Axin gene and its expression in glioma and explore the relationship between Axin gene and the occurrence of human glioma.Methods The point mutations of exon 8,9,10 of Axin gene were analyzed in 28 cases of glioma by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) analysis, silver staining and DNA sequencing. Immunohistochemistry was used to detect the Axin expression in these cases and normal brain tissues.Results Three missense point mutations were detected in exon 10 of Axin gene in 6 cases(21.4% ), in addition,3 silent mutations were detected in 3 cases; Eight cases of glioma(28.6%)showed positive Axin expression, and in the normal brain tissues Axin was expressed in neurons but not in gliocytes. There was one sample expressed Axin among those with point mutations.Conclusion Point mutations of Axin gene may be involved in the occurrence of glioma in human. |
| Keywords: | Axin Glioma PCR Immunohistochemstry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |