| 用两次聚合酶链反应检测血清中丙型肝炎病毒核糖核酸 |
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| 引用本文： | 侯淑霞,司聚同,王志新,孙德贵,王丹华,庄辉. 用两次聚合酶链反应检测血清中丙型肝炎病毒核糖核酸[J]. 中国公共卫生学报, 1992, 0(2) |
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| 作者姓名： | 侯淑霞 司聚同 王志新 孙德贵 王丹华 庄辉 |
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| 作者单位： | 北京医科大学公共卫生学院，北京师范大学生物系，北京医科大学公共卫生学院，河北省固安县卫生防疫站，北京医科大学公共卫生学院，北京医科大学公共卫生学院 100083，100083，100083，100083 |
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| 摘 要： | 应用丙型肝炎病毒核酸序列中的两对引物F-1(1—21),R-1(324—304),F-3(28—48),R-3(284—264),对4名抗-HCV阳性的献血员血清进行了两次聚合酶链反应("nested"PCR),经电泳鉴定1例为阳性,核酸长度为257bp。这一方法的建立为我国开展丙型肝炎病毒核糖核酸(HCV RNA)的检测提供了可能性。
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| 关 键 词： | 两次聚合酶链反应 HCV |
Detection of HCV RNA in Serum by "Nested" Polymerase Chain Reaction |
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| Hou Shuxia,School of Public Health in Beijing Medical University. Detection of HCV RNA in Serum by "Nested" Polymerase Chain Reaction[J]. , 1992, 0(2) |
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| Authors: | Hou Shuxia School of Public Health in Beijing Medical University |
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| Abstract: | An assay of nested polymerase chain reaction for detection of hepatitis C virus RNA in serum was established. Two pairs of primers in hepatitis C viral sequences, F-1(1-21), R-1(324-304) and F-3(25-48), R-3(284-264) were used. Of 4 anti-HCV positive serum samples of blood donors tested, one was positive for HCV RNA by the assay of "nested" PCR. The size of the PCR products observed on the gel electrophoresis was 257bp. The preliminary data "suggest the possibility of developing the nested PCR for detection of HCV RNA in serum |
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| Keywords: | " Nested" polymerase chain reaction Hepatitis C virus |
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