HMGB-1联合MSCs移植对急性心肌梗死大鼠心脏功能影响的实验研究

目的 研究高迁移率组蛋白-1(HMGB-1)联合骨髓间充质干细胞(MSCs)移植对于大鼠发生急性心肌梗死后心功能的影响及其相关作用机制.方法 将144只雄性SD大鼠分为健康对照组、模型对照组、MSCs移植组、HMGB-1注射组、HMGB-1注射+MSCs移植组及HMGB-1 BoxA注射+MSCs移植组等6组,术后第28天对大鼠的心脏功能、心肌的病理切片、心肌梗死的面积及梗死区新生血管的密度进行检测.并在心肌梗死术后第3、7、28天测定血清中相关细胞因子的水平.结果 在术后第28天,HMGB-1注射+MSCs移植组较其余5组的左心室舒张末期内径和左心室收缩期内径明显缩小,左心室短轴缩短率和射血分数明显增加(P＜0.05);HMGB-1注射+MSCs移植组梗死面积与其余模型组相比明显缩小(P＜0.05),梗死区的新生血管数目显著增加(P＜0.05).在术后第3天和第7天,HMGB-1注射+MSCs移植组的大鼠血清TLR4、VEGF水平最高(P＜0.05);术后第7天及第28天,HMGB-1注射+MSCs移植组大鼠的血清中白细胞介素6、核因子Kappa及肿瘤坏死因子α的水平最低(P＜0.05).结论 通过使用HMGB-1注射联合MSCs移植的治疗方法可以有效改善心肌梗死预后.

Experiment of effects of HMGB-1 combined with MSCs transplantation on cardiac function in rats with acute myocardial infarction

Objective To investigate the effects and mechanisms of HMGB-1 combined with MSCs transplantation on the heart function in rat with acute myocardial infarction.Methods A total of 144 male SD rats were divided into the healthy control group,model control group,MSCs transplantation group,HMGB-1 injection group,HMGB-1 injection+ MSCs transplantation group,HMGB-1 BoxA injection+MSCs transplantation group.On the 28th day after surgery,the heart function,myocardial pathological section,myocardial infarction area and new vessel density in infarction area were detected.Andthe level of related serum cytokines were measured on the 3rd,7th and 28th days after surgery.Results On the 28th day after surgery,left ventricular end diastolic dimension (LVDd) and left ventricular iiaternal diameter at end-systole (LVDs) in the HMGB-1 injection+ MSCs transplantation group were significantly decreased and the fractional shortening (FS) and ejection fraction (EF) value were significantly increased compared with the other five groups (P＜0.05);the infarction area in the HMGB-1 injection+MSCs transplantation group was significantly decreased and the new vessels number in the infarction area was significantly increased compared with the other model groups (P＜0.05).On the 3rd and 7th days after surgery,serum TLR4 and VEGF levels in the HMGB-1 injection+MSCs transplantation group were the highest (P＜0.05).On the 7th and 28th days after surgery,the levels of serum IL-6,NF-κB and TNF-α in the HMGB-1 injection+MSCs transplantation group were the lowest among all groups (P＜0,05).Conclusion HMGB-1 injection combined with MSCs transplantation treatment can effectively improve the prognosis of myocardial infarction.