Excretory/secretory antigen of Toxocara canis: recognition profiles of polyclonal and larvicidal monoclonal antibodies |
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Authors: | C DEL AGUILA C CUELLAR JL GUILLÉN |
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Institution: | Departamento de Parasitología, Facultad de Farmacia, Universidad Computense de Madrid, 28040 Madrid;Departamento de Biologia Molecular, fnstituto Llorenle-Fides, Carretera del Pardo Km. 1, 28035 Madrid, Spain |
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Abstract: | Summary Five monoclonal antibodies against the excretory/secretory (E/S) antigen of Toxocara canis were obtained and characterized. Immunoprecipitating activity was demonstrated in an in-vitro micropre-cipitating assay using live T. canis larvae. Their capacity to kill larvae was also shown in an in-vitro assay. Two zones of reactivity were observed in 7.5 and 12.5% SDS-PAGE (177-77 kD, 43-15 kD) of immunoprecipitates of human and mouse positive polyclonal anlisera. The murine monoclonal antibodies showed a common pattern of reactivity with the proteins in the 177-77 kD range. |
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Keywords: | Toxocara canis excretory/secretory antigen monoclonal antibodies |
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