| 高效液相色谱-蒸发光散射检测器法测定甘桔冰梅片中桔梗皂苷D含量 |
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| 引用本文： | 彭善贵,文永盛.高效液相色谱-蒸发光散射检测器法测定甘桔冰梅片中桔梗皂苷D含量[J].中国药业,2012(19):28-29. |
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| 作者姓名： | 彭善贵 文永盛 |
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| 作者单位： | 四川I省成都市食品药品检测中心,四川成都610045 |
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| 摘 要： | 目的建立测定甘桔冰梅片中桔梗皂苷D含量的高效液相色谱-蒸发光散射检测器法。方法色谱柱为DiamonsilC，。柱（200mm×4．6mm，5μm），流动相为乙腈-水（26：74），流速为1．0mL／min；Waters2424型蒸发光散射检测器，漂移管温度为85℃，气体流速为2．9SLPM／min。结果桔梗皂苷D进样量在0．952-11．900μg范围内与峰面积线性关系良好（r=0．9992），平均回收率为98．82％，RSD=1．74％（n,=6）。结论所建立的方法测定甘桔冰梅片中桔梗皂苷D的含量准确、可靠、操作简便。
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| 关 键 词： | 甘桔冰梅片 高效液相色谱一蒸发光散射检测器法：桔梗皂苷D |
Determination of Kikyosaponi D in Ganjie Bingmei Tablets by HPLC- ELSD |
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| Pertg Shangui,Wen Yongsheng.Determination of Kikyosaponi D in Ganjie Bingmei Tablets by HPLC- ELSD[J].China Pharmaceuticals,2012(19):28-29. |
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| Authors: | Pertg Shangui Wen Yongsheng |
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| Institution: | (Chengdu Municipal Food and Drug Testing Center, Chengdu, Sichuan, China 610045) |
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| Abstract: | Objective To determine the kikyosaponin D content in Ganjie Bingmei Tablets by HPLC evaporative light scattering detector （ELSD） method. Methods The analytical column was Diamonsil Cls column （200 mm x 4.6 mm, 5 μm）, with acetonitrile water（26 ： 74） as the mobile phase at the flow rate of 1.0 mL/min. The Waters 2424-ELSD was adopted for detection. The drift tube temperature was 85 ℃ and the gas flow rate was 2.9 SLPM/min. Results The sample size of kikyosaponin D in range of 0.952-11. 900 μg showed good linear relationship （ r = 0. 999 2）. The average recovery rate was 98.82%, RSD = 1.74% （ n = 6）. Conclusion This method for determining the kikyosaponin content is accurate, reliable and easy to operate. |
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| Keywords: | Ganjie Bingmei Tablets HPLC - ELSD kikyosaponin D |
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