Dynamic gut microbiome changes following regional intestinal lymphatic obstruction in primates |
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| Affiliation: | 1. Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center Shreveport, Shreveport, LA, USA;2. Department for General-Visceral and Transplant Surgery, University of Münster, Münster, Germany;3. University of Louisiana at Lafayette, New Iberia Research Center, New Iberia, LA, USA;4. Louisiana State University School of Medicine, Department of Gastroenterology and Hepatology, Shreveport, LA, USA;5. Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, Shreveport, LA, USA;6. Research Core Facility, Louisiana State University Health Sciences Center Shreveport, Shreveport, LA, USA;7. Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center Shreveport, Shreveport, LA, USA;8. Keeling Center for Comparative Medicine and Research, The University of Texas MD Anderson Cancer Center, Texas, TX, USA;9. Department of Pediatrics, Section of Pediatric Infectious Diseases, Louisiana State University Health Sciences Center - Shreveport, Shreveport, LA, USA;10. Department of Computer Sciences, Louisiana State University-Shreveport, Shreveport, LA, USA;1. Department of Physiology, Faculty of Basic Medical Science, University of Ibadan, Ibadan, Oyo, Nigeria;2. Department of Physiology, School of Health and Health Technology, Federal University of Technology, Akure, Ondo, Nigeria;1. Department of Biophysics, Faculty of Medicine, Cumhuriyet Üniversity, Sivas, Turkey;2. Department of Histology & Embryology, Faculty of Medicine, Cumhuriyet Üniversity, Sivas, Turkey;3. Department of Biochemistry, Faculty of Science, Cumhuriyet University, Sivas, Turkey;4. Department Of Physiology, Faculty of Medicine, Cumhuriyet Üniversity, Sivas, Turkey;5. Department of Neurosurgery, Faculty of Medicine, Cumhuriyet Üniversity, Sivas, Turkey;6. Department of Anatomy, Faculty of Medicine, Cumhuriyet Üniversity, Sivas, Turkey;1. Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria;2. Department of Clinical Pathology, Faculty of Clinical Sciences, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria;3. Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria;1. Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, Kyiv, Ukraine;2. Liberty University College of Osteopathic Medicine, 306 Liberty View Lane, Lynchburg, VA 24502, USA;1. Laoratories Technology Department, Turabah University College, Turabah, Taif University, Saudi Arabia;2. Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Egypt;3. Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Egypt;4. Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt;5. Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt;6. Chemistry Department, Faculty of Science, Turabah Branch, Taif University, Saudi Arabia;7. Faculty of Veterinary Medicine, Sadat City University, Sadat City, Menoufia, Egypt;1. Laboratory for Reproductive Biology and Developmental Programming, Department of Physiology, Edo University Iyamho, Edo State, Nigeria;2. Department of Physiology, Bingham University Karu, Nasarawa State, Nigeria;3. Department of Human Anatomy, Rivers State University, Port Harcourt, Nigeria;4. School of Medicine and Allied Health Sciences, University of the Gambia, Gambia;5. Institute of Chemical and Biotechnology, Vaal University of Technology, Southern Gauteng Science and Technology Park, Sebokeng, South Africa;6. Reproductive Biomedicine and Natural Product Lab, Department of Zoology, University of Rajasthan, Jaipur, 302004, India |
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| Abstract: | The pathogenesis of inflammatory bowel disease (IBD) has been linked with lymphostasis, but whether and how lymphatic obstruction might disturb the intestinal microbiome in the setting of Crohn’s Disease (CD) is currently unknown. We employed a new model of CD in African Green monkeys, termed ‘ATLAS’ (African green monkey truncation of lymphatics with obstruction and sclerosis), to evaluate how gut lymphatic obstruction alters the intestinal microbiome at 7, 21 and 61 days. Remarkable changes in several microbial sub- groupings within the gut microbiome were observed at 7 days post-ATLAS compared to controls including increased abundance of Prevotellaceae and Bacteroidetes-Prevotella-Porphyromonas (BPP), which may contribute to disease activity in this model of gut injury. To the best of our knowledge, these findings represent the first report linking lymphatic structural/gut functional changes with alterations in the gut microbiome as they may relate to the pathophysiology of CD. |
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| Keywords: | Inflammatory bowel disease Crohn’s disease Non-human primate Lymphatics Microbiome Lymphatic obstruction |
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