| RANKL/RANK通路介导神经母细胞瘤细胞迁移的机制探讨 |
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| 引用本文： | 王国文1,张 喆1,吕海欣1,崔丙周1,赵红宇2. RANKL/RANK通路介导神经母细胞瘤细胞迁移的机制探讨[J]. 现代肿瘤医学, 2019, 0(7): 1118-1121. DOI: 10.3969/j.issn.1672-4992.2019.07.005 |
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| 作者姓名： | 王国文1 张 喆1 吕海欣1 崔丙周1 赵红宇2 |
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| 作者单位： | 1.郑州人民医院，河南 郑州 453000;2.中国医科大学附属盛京医院，辽宁 沈阳 110004 |
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| 基金项目： | National Natural Science Foundation of China(No.81172410）；国家自然科学基金资助项目(编号:81172410) |
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| 摘 要： | 目的:探讨RANKL/RANK通路对神经母细胞瘤（neuroblastoma，NB）SH-SY5Y细胞系细胞侵袭和转移的作用机制。方法:通过pcDNA3.1+-RANKL和siRNA-RANKL转染NB SH-SY5Y细胞系过表达或沉默外源基因RANKL；细胞增殖实验(cell counting kit-8，CCK-8)检测外源基因RANKL转染NB SH-SY5Y细胞系对细胞增殖的影响；划痕试验检测外源基因RANKL转染NB SH-SY5Y细胞系对细胞迁移的影响；Western blot实验检测外源性基因RANKL转染NB SH-SY5Y细胞系后细胞内基质金属蛋白酶9（matrix metalloproteinase 9，MMP9）、基质金属蛋白酶2（matrix metalloproteinase 2，MMP2）表达变化。结果:通过pcDNA3.1+-RANKL和siRNA-RANKL对NB SH-SY5Y细胞系转染外源性RANKL基因后三组NB细胞增殖能力无统计学差异；RANKL基因过表达后NB SH-SY5Y细胞迁移能力增强（P<0.01），RANKL沉默后NB SH-SY5Y细胞迁移能力减弱（P<0.01）；RANKL基因过表达后NB SH-SY5Y细胞内MMP9、MMP2表达增强（P<0.05、P<0.001），RANKL沉默后NB SH-SY5Y细胞内MMP9、MMP2表达减弱（P<0.05、P<0.01）。结论:RANKL/RANK通路介导NB细胞迁移，并可能通过抑制细胞内MMP2、MMP9表达实现。
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| 关 键 词： | 神经母细胞瘤 RANKL/RANK通路 细胞迁移 基质金属蛋白酶9 基质金属蛋白酶 2 |
Mechanism of neuroblastoma cell migration invasion by RANKL/RANK signaling pathway |
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| Wang Guowen1,Zhang Zhe1,Lv Haixin1,Cui Bingzhou1,Zhao Hongyu2. Mechanism of neuroblastoma cell migration invasion by RANKL/RANK signaling pathway[J]. Journal of Modern Oncology, 2019, 0(7): 1118-1121. DOI: 10.3969/j.issn.1672-4992.2019.07.005 |
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| Authors: | Wang Guowen1 Zhang Zhe1 Lv Haixin1 Cui Bingzhou1 Zhao Hongyu2 |
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| Affiliation: | 1.People's Hospital of Zhengzhou，Henan Zhengzhou 453000，China；2.Shengjing Hospital of China Medical University，Liaoning Shenyang 110004，China. |
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| Abstract: | Objective:The aim of this study was to investigate the mechanism of cell migration activities on NB SH-SY5Y.Methods:To evaluate possible contributions of RANKL/RANK axis to cell invasion of neuroblastoma，the over expression vectors pcDNA3.1+-RANKL and knock down siRNA-RANKL were transiently transfected into SH-SY5Y cells.The cell growth was evaluated by cell counting kit-8（CCK-8) assay.The transfected-cells of SH-SY5Y migration were analyzed by wound healing assay.Expression of matrix metalloproteinase 9（MMP9），matrix metalloproteinase 2（MMP2） in transfected cells of SH-SY5Y was detected by Western blot assay.Results:To evaluate possible contributions of RANKL/RANK signaling pathway to cell invasion/migration of neuroblastoma，the over expression vectors pcDNA3.1+-RANKL and knock down siRNA-RANKL were transiently transfected into SH-SY5Y cells.At first，We demonstrated that RANKL/RANK signaling pathway had no effect in SH-SY5Y proliferation.Moreover overexpression of RANKL accelerated the cell migration，meanwhile increased the expression of MMP9，MMP2（P<0.05，P<0.001），whereas knockdown of RANKL was quite the contray（P<0.05，P<0.01）.Conclusion:The effect of cell invasion and migration were inhibited by RANKL/RANK signaling pathway via down-regulation expression of MMP9 and MMP2. |
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| Keywords: | neuroblastom RANKL/RANK cell migration matrix metalloproteinase 9 matrix metalloproteinase 2 |
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