人参皂苷Rg3差异调节前列腺癌细胞PC3和DU145增殖

目的 探讨人参皂苷Rg3对前列腺癌细胞PC3和DU145细胞增殖的调节作用。方法 100 µmol/L人参皂苷 Rg3处理体外培养的前列腺癌细胞 PC3和 DU145，使用 CCK-8实验检测细胞的增殖能力；DCFH-DA活性氧荧光探针试剂盒检测人参皂苷 Rg3处理的 DU145细胞中活性氧（ROS）水平；JC-1法检测 PC3和 DU145细胞线粒体膜电位的去极化；RT-PCR和免疫印迹法检测PC3和DU145细胞中氧化还原相关蛋白的表达差异。结果 CCK-8实验结果表明，100 µmol/L人参皂苷 Rg3能够显著抑制 PC3细胞增殖，而对 DU145细胞的增殖没有显著调节作用；DCFHDA活性氧荧光探针试剂盒检测结果表明，人参皂苷 Rg3能够显著上调 DU145细胞和 PC3细胞中 ROS水平；JC-1检测实验表明，人参皂苷Rg3能够诱导PC3和DU145细胞中线粒体膜电位去极化；RT-PCR和免疫印迹实验表明，抗氧化蛋白谷胱甘肽过氧化物酶-1（GPX-1）和超氧化物歧化酶2（SOD2）在DU145细胞中的表达显著高于PC3细胞。结论 人参皂苷Rg3对PC3和DU145细胞的增殖表现出不同的调节作用，可能与细胞中抗氧化蛋白的表达差异有关。

Differential regulatory effects of ginsenoside Rg3 on the proliferation of prostate cancer cell lines PC3 and DU145

Objective To explore the different regulatory effects of ginsenoside Rg3 on the proliferation of prostate cancer cells PC3 and DU145. Methods PC3 and DU145 cells were cultured in vitro and treated with ginsenoside Rg3 at 100 µmol/L. Then CCK-8 assay was performed to evaluate the proliferation of cells. Reactive oxygen species (ROS) levels were detected using DCFH-DA staining in DU145 cells treated with DMSO or ginsenoside Rg3. JC-1 staining was performed to investigate the depolarization of mitochondrial membrane in PC3 and DU145 cells treated with ginsenoside Rg3. Differential expression of antioxidant proteins in PC3 and DU145 cells were analyzed by RT-PCR and Western blot assay. Results CCK-8 assay showed that the proliferation of PC3 cells was significantly inhibited by 100 µmol / L ginsenoside Rg3 while no inhibitory effect was observed on DU145 cells. Results of DCFH-DA staining assay showed that the ROS levels in DU145 cells and PC3 cells were up-regulated by ginsenoside Rg3. Furthermore, results of JC-1 assay demonstrated that ginsenoside Rg3 induced the depolarization of mitochondrial membrane both in PC3 and DU145 cells. The results of RT-PCR and Western blot assay indicated that the expressions of glutathione peroxidase-1 (GPX-1) and superoxide dismutase 2 (SOD2) were higher in DU145 cells compared to those of PC3 cells. Conclusion Ginsenoside Rg3 shows different regulatory effects on the proliferation of PC3 and DU145 cells, which may be related to the differential expression of antioxidant proteins in the two cell lines.