应用二维电泳法对视网膜色素上皮细胞光损伤后蛋白质组学的初步研究

目的 应用二维电泳法观察光损伤后视网膜色素上皮细胞蛋白质组的表达。 方法 运用冷白光以（2200±300）Lx光照人视网膜色素上皮细胞(ARPE 19)6 h，建立光损伤模型；提取细胞可溶性蛋白并通过二维电泳分离和凝胶图像分析，寻找光损伤细胞的蛋白质谱变化。 结果 软件分析显示全细胞可溶性蛋白在图谱上出现（390±10）个清晰斑点,11个蛋白斑点有明显表达差异。光损伤细胞内8种蛋白表达上调,1种下调，2种蛋白表达缺失。 讨论 二维电泳实验能够找出光损伤RPE细胞与正常细胞的蛋白表达差异，为进一步研究在此改变中发挥重要作用的蛋白质奠定基础。

A preliminary study on the expression of proteins in light-injured retinal pigment epithelial cells by two-dimensional electrophoresis

Objective To observe the expression of proteins in light-injured retinal pigment epithelial (RPE) cells. Methods ARPE 19 cells were exposed to the cool white light at the intensity of (2200±300) Lx for 6 hours to set up the light injured model. Cellular soluble proteins was extracted and analyzed by means of two dimensional electrophoresis to find out the changes of protein map of light injured RPE cells. Results Cellular soluble proteins had (390±10) spots on the map, in which 11 spots had obvious difference between the light injured group and the normal control group. In the light injured cells, the expressio of 8 proteins increased, 1 decreased, and 2 disappeared. Conclusion Two dimensional electrophoresis can find out the difference of expression of proteins in light injured and normal RPE cells.