镁金属纤维丝与雪旺细胞共培养时细胞增殖及神经生长因子的表达

目的：在镁金属纤维丝与雪旺细胞共培养下,探讨微环境下的镁离子是否对雪旺细胞增殖及神经生长因子（nerve growth factor,NGF）分泌有影响作用.方法：分离、培养雪旺细胞,选取第2代细胞,调整浓度以1×108 L-1接种于培养皿,并随机将雪旺细胞分为2组.A组：加入一段1cm长的镁金属纤维（AZ31镁合金（Mg-3％Al-1％Zn））丝于培养皿中联合培养,B组：对照组,单纯雪旺细胞培养.在光镜、电镜下对雪旺细胞细胞形态学观察.分别于2,4,6,8,10和12天,MTT法检测雪旺细胞增殖的情况,ELISA检测细胞培养上清中神经生长因子的含量.结果：MTT法测得在培养第6-8d时,实验组OD值明显高于对照组OD值（P＜0.05）,ELISA法测得,两组中的神经生长因子的表达均随时间的增长而升高,虽对照组进入峰值的时间过早,但在第6-8d,NGF的表达明显高于对照组（P＜0.05）.结论：微环境下,镁离子可以促进细胞组织黏附及分泌生长因子NGF作用.

Effect of Schwann cells proliferation on co-culture with magnesium

Objective： To investigate the effect Mg＋ on Schwann cells culture, and to analyze the biocompatibility of Schwann cell co-culture with magnesium, Methods： Wallerian degenerated sciatic nerves were harvested from adult rat. Schwann cells were cultured by enzymatic digestion and differential adhesion methods. We prepare experiment group which I x 10^8/L cells culture was then seeded onto the Mg as well as petri dish. And the experiment group was only Schwann cells.MTT was used to observe the viability of the Schwann cells at days 2, 4, 6, 8, l0 and 12day, respectively. The expression of NGF in Schwann cells was detected by ELISA at the same time point as MTT. Results： MTT coloritric assay revealed that significant higher OD value peak of 6-Sday in Mg elevated compared with control group on the seventh day （P〈0.05）.NGF had a similar expression pattern with one peak either in the co-cultured Schwann cells or Schwann cells cultured alone. However, compared with the Schwann cells cultured alone, the expression levels of NGF mRNA of experiment froup were significant higher than the experiment group.Conclusion： These findings suggest that Mg＋ is capable of regulating the biological behaviors of Schwann cells, including proliferation,cell adhesion, spreading, as well as the expression and secretion of NGF.