黄曲霉毒素G1对体外培养HPBM增殖及TNF-α分泌的影响

目的:研究黄曲霉毒素G₁(AFG₁)对体外培养人外周血单个核细胞(HPBM)增殖及细胞TNF-α分泌的影响。方法:采用流式细胞术(FCM)和MTT比色法研究AFG₁对HPBM增殖的影响。以双抗体夹心ELISA法检测AFG₁对HPBMTNF-α分泌的影响。结果:FCM检测结果显示,AFG₁作用6h,1000μg/L处理组HPBM的增殖指数明显高于对照组。AFG₁作用24h,200μg/L和1000μg/L浓度的AFG₁可明显刺激HPBM增殖;回归分析结果表明,AFG₁作用6h和24h,AFG₁浓度均与增殖指数呈正相关(r分别为0.5122和0.5119,P均＜0.05)。MTT比色法结果显示,2000μg/LAFG₁处理HPBM的A值明显高于对照组。AFG₁在100μg/L浓度下可显著抑制TNF-α分泌(P＜0.05)。结论:AFG₁对体外培养HPBM的增殖有刺激作用,在100μg/L浓度下对HPBMTNF-α的分泌有一定抑制作用。

Effects of aflatoxin G1on proliferation and TNF-

AIM: To explore the effects of aflatoxin G1(AFG1 )onproliferation and TNF-α secretion of human peripheral blood mononuclear cells(HPBM) in vitro. METHODS: The effects of AFG1 on proliferation of HPBM were analysed with flow cytometric (FCM) DNA analysis and MTT bioassay, while that on TNF-α secretion was detected with ELISA. RESULTS: FCM analysis revealed that 6 h after treatment, proliferation index(PI) of 1000 μg/L AFG1 treated HPBM was significantly higher than that of control. 24 h after AFG1 treatment, stimulating effects on proliferation was found in HPBM treated with AFG1 at 200 μg/L and 1 000 μg/L.Regression analysis showed that PI was postively correlated with the concentrations of AFG1 in the concentration range from 0 to 1 000μg/L( r=0. 5122 and 0.5119 respectively,P＜0.05).MTT bioassay showed that the A value of the cells treated with AFG1 at 2 000 μg/L was higher than that of the control. Double antibody sandwich enzyme linked immunosorbent assay (ELISA) results showed that AFG1 at a dose of 100 μg/L could significantly inhibit lipopolysaccharide-induced TNF-α secretion. CONCLUSION: AFG1 could stimulate the proliferation of HPBM and could decrease TNF-α secretion at certain concentration.